Tag Archives: confocal

Tuesday 2/14/12

Posted on February 14, 2012 by admin

10:00A  — 12:20 A  Flip fly cage, 10:00A confocal imaging of H2 (multi histone stains, slide 70) embryos to cut. Mark region imaged by confocal using dissecting scope in CBI. confocal images of S6, S7, H3, sections marked, thin-sectioned onto slides. … Continue reading →

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Monday 02/13/12

Posted on February 13, 2012 by admin

10:00A -7P, 10P-12:20A Visiting postdoc talk (Bryan). 10A-1P Flip fly cage 1P. Some STORM stacks didn’t process in batchinsightm.  Some issues still in running code, tweaked code and re-running missing data sets: S45, S44ubx, Pc, S45_13i. Working on image registration … Continue reading →

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Protected: H3K4me3 by confocal and STORM

Posted on February 12, 2012 by admin

There is no excerpt because this is a protected post.

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Sunday 02/12/12

Posted on February 12, 2012 by admin

9:45 A — 5:20P, 8:30P — 11:50P Finish STORM O/N of S45 (13-introns) setup STORM run of Pc Flip fly cage (10:50P) Flip fly cage (1:50P) Hal holds control of 647 laser AOTF against labview. Fixed. Analyzing STORM data by … Continue reading →

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Friday 02/09/12

Posted on February 10, 2012 by admin

10:10 A — 11:20 P move samples from 4C, change 100% EtOH STORM: setup 10x objective for finding samples. substantial offset, need to calibrate. Need to set laser powers to zero but have lasers on before starting dave! Ordered Bloomington … Continue reading →

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whole-mount tests 02/09-10/12

Posted on February 10, 2012 by admin

slide H1: 4 color anti-nuclear lamin test   slide H2: Directly labeled antibodies: Dm01-488(green) H3K27me3-Cy3B (red), H3-Cy5 (blue), H3K27ac-750 (not shown) slide H4: H3K27me3-Cy3B (red) and H3K4me3-Cy5 (blue) along with 405 background (green). slide H4: earlier embryo: H3K27me3 ‘clusters?’  (red) … Continue reading →

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Thursday 02/09/12

Posted on February 9, 2012 by admin

9:50 A – 1:00A Remove and keep primaries from all staining reactions (except traditional snail labeling of MP08 embryos) Washing embryos, in situs / staining reactions day 3. new stock of 1x PBT clean frozen fly cages Flip fly cage: … Continue reading →

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Wednesday 02/10/12

Posted on February 1, 2012 by admin

11:00A – 7:30P, 8:30P-11:30P Flip collection cages 11:20A Order Primers for Taf1, aTub84B, m13F/R Confocal: STORM prepped slides.  Both 1hr and 35 min samples are substantially over etched, most of sample is gone.  Try 20 min next (?). Confocal: MP08 … Continue reading →

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Saturday 01/28/12

Posted on January 28, 2012 by admin

11:00 A confocal O/N scans still running (22/26 embryos, started at 10 P, 35 sections, speed 5).  Extended run time until 3P. Literature search for good Drosophila Cohesin antibodies wrote to Dale Dorsett, might have Rad21 antibody to send, recontact … Continue reading →

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Friday 01/27/12

Posted on January 27, 2012 by admin

10:00A check 750 dye-efficiencies using STORM 2 lasers.  Detailed observations recorded on Embryo_staining database. multi-histone label sample 2 looked alright by conv imaging on STORM, even 488-ftz is detectable. New microtome installed in lab, training 12-2P. Oven did not reset … Continue reading →

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