Tag Archives: STORM

Monday 07/01/13

Posted on July 1, 2013 by admin

9:45 A – 8:00 P 9:00 P – 12:00 A STORM O/N STORM lost focus early in night :(. Reset focus, got a few more cells during 4.5 hour lab meeting. Replaced buffer on chiseled PhF slide. Setting up to … Continue reading →

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Thursday 05/06/13

Posted on June 6, 2013 by admin

10:30A – 7:30P, 9:00P-12:45A project 2: Team meeting, 2PM notes and planning Clarification meeting with XZ more cell staining Presentations putting rough slide drafts together for Wu lab presentation taking slides from non sensitive data for SDB presentation STORM STORM … Continue reading →

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Friday 05/31/13

Posted on June 1, 2013 by admin

10:00 A – 7:00P, 9:30P – 12:30 A Check RNA hybes on STORM2 (hybed at 50C (55C?) in dry oven O/N in coverglass preps like for DNA labeling) (quick check on turnkey looked promising) tub looks good but hard to … Continue reading →

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Tuesday 05/21/13

Posted on May 21, 2013 by admin

8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading →

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Sunday 05/19/13

Posted on May 19, 2013 by admin

10:30 A – 10:00 P, 11:00 P – 12:15 A Probe extraction Previous test gel, suggesting some samples at least have probe: Run denaturing gel at 100 uL scale 95 minutes. Cut out smallest band over 50 bp. sample loading … Continue reading →

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Wednesday 05/08/13

Posted on May 8, 2013 by admin

9:30 A — Probe making pool digests aliquot out test DNA: 1 uL digest DNA, 3 uL TBE, 1 uL 6x loading dye + 12x gel green, 5 uL formamide with 5% 5M EDTA Pour new 16% urea PAGE gels … Continue reading →

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Protected: Ph FLAG and Ph FLAG M

Posted on May 7, 2013 by admin

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Protected: issues with ph-FLAG round 1

Posted on April 5, 2013 by admin

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Friday 03/29/13

Posted on March 29, 2013 by admin

9:30 A – 8:10 P, 9:00 P – 12:10 A Install cryo-attachment be sure to retract arm and center FC mount prior to mounting be sure there is space between the arm and the chamber so the arm can move … Continue reading →

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Tuesday 03/12/13

Posted on March 12, 2013 by admin

10:00A – 11:15P STORM Finish O/N STORM run on STORM2 Finish O/N STORM run on STORM3 Installing mount and sliders on STORM4 to allow for 3D imaging Lessons: Final appeture (removed for alignment) front-back position chosen to minimize the size … Continue reading →

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