Tag Archives: coding

Thursday 03/07/2013

Posted on March 7, 2013 by admin

9:30 A – 11:10 P Coding Reorganizing Image_Analysis Repository New folder for 2013 Cell Reports paper Major rewrite of LSM parsing code And 6 hours later: LSMextract.m – Takes a .lsm file, extracts all z-positions and all stage-positions into multicolor … Continue reading →

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Tuesday 03/05/13

Posted on March 5, 2013 by admin

9:50 A – 7:30 P, 8:30 P – 10:50 P General Discussing replies for Banff invites. Student substitutions add to bottom of list Still need to write more acknowledgement of replies. STORM Analysis launch analysis of all unprocessed datafiles. ESATA … Continue reading →

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Monday 03/04/13

Posted on March 4, 2013 by admin

9:45 A – 7:30 P, 9:00 P-11:30 P General stuff Group meeting: see notes send out invites to Banff workshop Continue working on writing referee review Fly work flipped all bottle stocks tossed all Mat alpha tub bottles currently expanding: … Continue reading →

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Sunday 03/03/13

Posted on March 3, 2013 by admin

10:00A – 1:00P, 6:00P – 7:45P, 9:00P – 11:00P Embryo Section staining Control test with AATAT worked. basic protocol No 92C pre-denature 60C 25 min minimal cooling gap. Add probe to slide, drop coverglass on, seal 94C heat-block 2.5 min … Continue reading →

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Saturday 03/02/13

Posted on March 2, 2013 by admin

10:00A – 7:00P, 8:30P-10:30P Writing stuff Revised Banff Invites letter sent to co-organizers write to van Steensel about R code Review/referee article read clean through manuscript wrote up all critiques. To do: write up manuscript strengths and contributions to field. … Continue reading →

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Updating Web Notebook: version management

Posted on March 1, 2013 by admin

Methods for version management: WP Document Revisions adds a new tab to main side widget (below Pages and Comments), called Documents. Under this tab you can upload files from your computer. These now behave has posts. Instead of editing the … Continue reading →

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Friday 03/01/13

Posted on March 1, 2013 by admin

8:10 A – 8:10P, 9:30P – 11:00P Confocal helping scan Hazen’s sections Embryo section staining BXC DFISH NO BX-C staining detectable after post-hybe hot-wash (50-60C in waterbath, 25 min). Repeat staining, try short coldwash and then check cells for signal. … Continue reading →

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Thursday 02/28/13

Posted on February 28, 2013 by admin

10:20 A -6:45P, 8:15P – 11:55P (workout 8:30-10:20A) Probe Making (1P-6P @ Longwood) emPCR for 100kb yellow, green and black regions (at Wu lab) see new protocol 35-50 ng/uL yield (11 uL total volume) Notebook Looking in to version managing … Continue reading →

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Wednesday 02/27/13

Posted on February 27, 2013 by admin

9:00 A – 10:00 P Embryo collection Flipped Esc cage — good looking overnight collection Probe design schematic of probes design and send to Brian new probe sequences for Custom Array order. working on new probe sets for colors project … Continue reading →

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Sunday 03/24/13

Posted on February 24, 2013 by admin

11:30A – 5:00P, 6:30P – 8:15P Coding Overhauling all file loading for STORMrenderBeta Single Bin files Loaded through open menu (or toolbar) Multiple Bin files, manual select Prompts user to find a chromatic warp file Allows warp dimension to be … Continue reading →

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