Tag Archives: probe making

Friday 10/26/12

Posted on October 26, 2012 by admin

10:20 A – 7:30PM Fly Work  Set up collection cages for TRiP knockdown experiments: MTD x Psc 1 MTD x Pc 3 Set up new collection cage of MTD controls.  Pc labels looked weak and Pc in situs did not … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Friday 10/26/12

Thursday 10/25/12

Posted on October 25, 2012 by admin

9:50 A – 7:00P, 8:30P – 11:20 P Clean and coat coverslips washout fix from O/N fix samples, move to EtOH rehydrate, check for presence of staining. Respond to team about NSF BIRS proposal Pol I arrived 4:00 PM check … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Thursday 10/25/12

Monday 10/22/12

Posted on October 22, 2012 by admin

9:20 A – 8:30, 9:30 – 11:10PM Flip collection cage take embryos out of 4C, final hour incubating at RT before washes. snail in situs Draq5 stained and mounted STORM / section in situs / DFISH all in secondaries at … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Monday 10/22/12

Thursday 08/02/12

Posted on August 2, 2012 by admin

9:50A — 6:20, 8:20p – 11:55P Confocal O/N — not enough sections / .17 spacing too close to make it through nuclear layer.  trashed image. STORM O/N — Hal froze on movie 48.  Sent crash report to Hazen.  Shut down … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Thursday 08/02/12

Wednesday 08/01/2012

Posted on August 1, 2012 by admin

10:10 A – 7:30P, 8:20P – 10:45P Flip collection cages. Meeting with Ting and Xiaowei. KE miniprep cg5… cg10… and bxd samples.  lab colonies did not grow in liquid culture?  Sent samples for sequencing. STORM of Pc6_cntrl Label new dk-rb-750 … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , , | Comments Off on Wednesday 08/01/2012

Tuesday 07/17/12

Posted on July 17, 2012 by admin

10:10 A – 7:00P, 8:20 P – 11:10P working on presentation for SDB teaching primer design for KE: new primers for: bxd, lab, #scr, CG5520, CG3995, CG10326, CG10324.  Ordered. KE confocal of last weeks embryo staining in vitro transcription reactions … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 07/17/12

Tuesday 06/26/12

Posted on June 26, 2012 by admin

10:30 A -5:00P, 9:00P – 11:30P in situs day 2 short fix 2-14 hour yw and Pc embryos. rehydrate and stain 2-14 hour short fix embryos with Su(Z)12.  Pc-GFP embryos also stained with ch a-GFP. vball lost. Synthesized Bac 1 … Continue reading →

Posted in Summaries | Tagged , , , , , | Comments Off on Tuesday 06/26/12

Wednesday 05/09/12

Posted on May 9, 2012 by admin

12:30 P – 9:45 (volleyball practice 8:30A-11:30A) Pellet and resuspend all new probes. updated probe & plasmid database with new & resynthesized probes: [spreadsheet 0AjSqkxgziU1YdHlnZmFzOVBsX0JQUjlrQnRUWjVYVGc 600 400 sheet=1] 3x maternal Gal4 flies arrived from Perrimon lab -> start expanding coding molecule … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Wednesday 05/09/12

Tuesday 05/08/12

Posted on May 8, 2012 by admin

10:00A – 10:50 P Bcd Gal4 x shRNA Pc2 and x Psc shRNA1 both still hatch larvae. Flip collection cages check mail for Perrimon Gal4 3x line – not yet arrived rerunning Pc-GFP controls with Colenso’s parameters (min height 750 … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , , | Comments Off on Tuesday 05/08/12

Wednesday 04/25/12

Posted on April 25, 2012 by admin

9:45 A – 8:30 P O/N hybridization — should do in PCR block.  Water evaporates from 37C block, not good contact with PCR tube.  Refilled water, allow another hour of hybrdization before washes. Sequencing results:  A1 = Taf1 antisense (sequence with m13F … Continue reading →

Posted in probe and plasmid building, Summaries | Tagged , , | Comments Off on Wednesday 04/25/12