Tag Archives: STORM

Monday 03/11/13

Posted on March 11, 2013 by admin

10:00 A Group meeting (Eileen) see notes Journal club: High salt recruits aversive taste pathways STORM Hal crashed on STORM3 at 10:11A (still 34 locations acquired). sent STORM3 error report to Hazen (see email record) STORM2 run seemed to finish … Continue reading →

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Sunday 03/10/13

Posted on March 10, 2013 by admin

9:30 A – 12:15 A Meeting with Ajaz oops it’s daylight savings day! Ajaz will induce and fix cells, bring over fixed cells on coverglass label with m-anti-Flag + A647-A405-dk a-m secondary and STORM. Compare clusters. STORM Finished O/N imaging … Continue reading →

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Saturday 03/09/13

Posted on March 9, 2013 by admin

10:30 A STORM setup imaging of 2x-Cy5 labeled Fab7. Hardly detectable loci in conventional — many (most?) cells no candidate locus detectable. General cy5 background seems higher. Found a few cells that look like reasonable candidates for conventional. Background clearly … Continue reading →

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Tuesday 03/26/13

Posted on February 26, 2013 by admin

10:00 A – 6:30P, 7:50P – 10:20P Embryo work Flip collection cages ~10:00 A No hatching from Mat apha tub x Rad21, Scm, Pc(3), Psc(1) shRNAs. all these embryos appear unfertilized. Frequently moderate deformaties to egg shape, substantial variation in … Continue reading →

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Saturday 03/23/13

Posted on February 23, 2013 by admin

10:30 A – 6:20P, 9:30P Embryo collection Flip all fly cages dispose of previous fly plates with new plate recycling and freezing system. Fix embryos 9:30 PM (~3-14 hrs, mostly at raised at 29C). STORM working out parameters for TCEP … Continue reading →

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Wednesday 02/20/13

Posted on February 20, 2013 by admin

10:00 A – 7:15P, 8:30P – 11:15P STORM Samples: BXC cy5 (single primary) and BXC cy5 + 750 secondary BXC-cy5 mounted for imaging 12:00P Realigned optics Taking bead calibration images Working out parameters to do z-scan. Can’t go too far … Continue reading →

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Sunday 02/17/13

Posted on February 17, 2013 by admin

STORM Finish O/N run of BXC + flank try2 (3D this time). Did not lose TIRF. Did not overfill drive or crash computer (4GB left on hard-drive, 400 written). Copying to new Alistair10. (will move to the new Alistair9 when … Continue reading →

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Thursday 02/14/13

Posted on February 14, 2013 by admin

9:20 A -7:00P, 9:00P – 10:30P STORM Finish O/N confocal More cells not in TIRF – sections not bleaching, even 647. Dropped TIRF angle to 14.2, now switching well, still good contrast. Might need to re-image this slide later today. … Continue reading →

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Friday 02/08/13

Posted on February 9, 2013 by admin

11:00 A – 8:30P, 9:30P-11:00P (remote) Fly work collect virgins non-Sp CyO males of Esc not obviously extra sex combs (only 1 male so far). Need to check bristly number and frequency expected (see Vivek’s paper). new bottles of MTDs … Continue reading →

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Wednesday 02/06/13

Posted on February 6, 2013 by admin

9:00 A – 8:30P, 9:30P-11:00P Mulitplex STORM covergalss shattered en route from HMS to cambridge, extracting fragments of slides, successfully ID Bx-C Pc. stained BX-C with Pc-488 (new ab-cam anti-rb). Stained additional Pc labeled slide with Pc-750. (1 hr + … Continue reading →

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