Tag Archives: STORM

Coding Goals 02/02/13

Posted on February 3, 2013 by admin

Short Term Goals DaoSTORM Clean up calls for DaoSTORM in STORMfinder to use simplier DaoSTORM installation. (Complete 02/02/13) Test run DaoSTORM Merge all active projects in Matlab_STORM into Beta Move latest version of Insight3 and DaoSTORM in matlab-storm. (completed 02/02/13) … Continue reading →

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Saturday 02/02/13

Posted on February 2, 2013 by admin

11:30 A – 1P, 1:45P – 12:45 A STORM Overnight run on STORM3 crashed at around 34 of 85 movies — ‘memory error’. send crash report to Hazen. copying data form Alistair9 to Alistair8. Imaging BX-C embryos with H3K27me3. One … Continue reading →

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Friday 01/02/2013

Posted on February 1, 2013 by admin

9:45 A – 7:15P, 8:30P – 10:40 P STORM Record z-calibration curve for STORM3 Finish O/N run of Fab7 on STORM3 Finish O/N run of BX-C embryos on STORM2. Ideal focus plane not matched between regions (this can be custom … Continue reading →

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Thursday 01/31/13

Posted on January 31, 2013 by admin

10:45 A – 12:30 A (8:45-10:45 workout) embryo section labeling quick check after 10 min hot wash in incubator at 70C in SSCT, found no sections. DAPI staining sections. Try again. weak BXC detectable: Prepped for STORM TRY 2: prepping … Continue reading →

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Thursday 01/24/13

Posted on January 24, 2013 by admin

9:30 A – 7:30P STORM * Finish O/N run by 10A. * Run Z-calibration * Copy and delete data!  Remove external hard-drive * Image BX-C + H3K27me3-488 slide (at 4C in PBT).  maybe we should store these in 2x SSCT? … Continue reading →

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Wednesday 01/23/13

Posted on January 23, 2013 by admin

10:30 A – 8:30P, 9:30P – 11:45P (8:45-10:30A workout) Fly Work collect virgins: Df/CyO,esc[2],  2x mat-tub Gal4. collect males: esc[6]/Sp. cross (1 new bottle) esc[6]/Sp x Df/CyO,esc[2] (should have used Gla, will need to screen Sp for all flies that … Continue reading →

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Friday 01/10/13

Posted on January 11, 2013 by admin

8:30 A – 9:50 P Fly work Flip collection plate 8:30A Fixing embryos 11:30A.  (20 min 8% FA reaction) Flip all polycomb mutants into bottles STORM made new bead slide, 1:25K imaging 2 rounds of beads for 3D fitting K9me2 … Continue reading →

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Thursday 01/10/13

Posted on January 10, 2013 by admin

8:30A – 10:30P Flip collection plate 8:30A Fixing embryos 10:30A.  (20 min 8% FA reaction) O/N confocal runs — 700 at speed 4 still running.  5 not quite as clean. copy data. Make new fly plates (~4 tubes of plates). … Continue reading →

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Wednesday 12/19/2012

Posted on December 19, 2012 by admin

10:30 A – 6:00P Testing HP1 STORM imaging.  647 switching extremely well, extremely dense movies once optics properly aligned, no 405 required Had 405 base power ~30 mW instead of 1.5, might have bleached out 750 movies. testing 488 +/- … Continue reading →

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Thursday 12/13/12

Posted on December 13, 2012 by admin

10:15 A -7:00P, 8:00P – 9:45P, STORM imaging TIRF controller crashed.  Will not revive. After hours of various attempts and help from several lab members, Graham finds in manual that power cycling the controller fixes the problem.  Unplug the black … Continue reading →

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