Tag Archives: cell labeling

Thursday 11/23/12

Posted on November 21, 2012 by admin

10:30 A – 7:30 P Thanksgiving Fly work expand YWs Flip stocks check crosses.  double HbZ not yet emerging.  recombineering crosses looking bleak, we’ll see in anotehr 5-6 days… Probe making New library stats spin down dissolving probe.  Gently Pippett … Continue reading →

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Tuesday 11/20/12

Posted on November 20, 2012 by admin

9:45 A – 11:30 P Confocal Test protein stain (didn’t look good in conventional images) H3K27me3 weakly detectable. H3K9me2 not stained. Psc, SuZ(12), not well stained. compared to DFISH S2 cell (slide #1 in record, 10/23)  All channels (except 1:100 … Continue reading →

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Monday 11/19/12

Posted on November 19, 2012 by admin

9:15 A — 7:15 P, 8:15P – 1:15 A Cell labeling hot washes for probe first slide (2x at 60C) 1x at RT remove and save primary antibodies at RT for primary first slide. PBT rinses (3x soln change, 3rd … Continue reading →

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Sunday 11/18/12

Posted on November 18, 2012 by admin

10:15 A – 6:50 P, 7:50P – 10:30P Probe making have not confirmed nicking enzyme concentration for digestion.  Canceled PAGE introduction with Bryan, will attempt again later. Telomeres Read over telomere manuscript from John Wu  Imaging telomeres: 656 = 60 … Continue reading →

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Saturday 11/17/12

Posted on November 17, 2012 by admin

10:00 A – 12:00P, 3:30P – 8:00P, 9:00P-11:20P S2 Cell cent + chromatin/PcG Hot washes PBT washes block 4 hrs antibodies: H3K9me2, Pc, PCNA (Fisher) (1:3 across the top) rinse, block, secondaries O/N at 4C Harvard wins Yale Game Chromatic … Continue reading →

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Friday 11/16/12

Posted on November 16, 2012 by admin

9:45 A – 7:00P, 8:00P – 10:30P Making summary images of SCM data. Reconcentrating / Precipitating probe prior to nicking reaction. Combined into 2 tubes. Dissolving pellets at 37 O/N.  (waiting for Nicking enzyme to continue). Nicking enzyme arrived Brian … Continue reading →

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Saturday 11/03/12

Posted on November 3, 2012 by admin

10:45 A – Writing Write BJ review Drosophila PWMs TF binding motifs FlyFactor Ecoli TF binding + PWMs here (might be something better) Finish first draft through of enhancer integration section.  On to promoters next. DFISH on cell culture remove … Continue reading →

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Tuesday 10/30/12

Posted on October 30, 2012 by admin

9:30 A – 7:30P, 8:30P – 11:50 P Flies Flip collection plates. # Save MTDs to plate into new bottles.  All in one, need high density. Collect virgin Espl/sim, cross to TM2/TM6 males. Collect virgin Act5-gal4 Make new AJ plates … Continue reading →

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Saturday 10/27/12

Posted on October 27, 2012 by admin

10:30A – 8:ooP Stop O/N confocal by 11A. Confocal data check: Late positions start too low.  Not sure why — maybe middle position to close to edge changed microscope positions? Fly Work Collect virgin Espl[D]/ sim[D].   Flip fly stocks … Continue reading →

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Tuesday 10/23/12

Posted on October 23, 2012 by admin

9:20 A – 6:30P, 8:00P-11:00P (remote) S2 cell DNA-FISH labeling Fixing S2 cells for DFISH (1/2x PBT 5% formaldehyde) cells still attached, looks promising. cells preincubated at 92C for 3min, 60C for 20min, moved to RT probes denatured at 92C … Continue reading →

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