May 2024 M T W T F S S « Aug 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Categories
- AP patterning (13)
- Blog (1)
- Chromatin (88)
- Conference Notes (72)
- Fly Work (54)
- General STORM (25)
- Genomics (134)
- Journal Club (22)
- Lab Meeting (66)
- Microscopy (79)
- Notes (1)
- probe and plasmid building (58)
- Project Meeting (3)
- Protocols (13)
- Research Planning (74)
- Seminars (21)
- Shadow Enhancers (59)
- snail patterning (40)
- Software Development (5)
- Summaries (1,412)
- Teaching (9)
- Transcription Modeling (40)
- Uncategorized (10)
- Web development (19)
Links
Tags
analysis cell culture cell labeling chromatin cloning coding communication confocal data analysis embryo collection embryo labeling figures fly work genomics hb image analysis image processing images in situs Library2 literature making antibodies matlab-storm meetings modeling MP12 mRNA counting Ph planning presentation probe making project 2 project2 result results sectioning section staining shadow enhancers sna snail staining STORM STORM analysis troubleshooting writing-
GitHub Projects
Tag Archives: chromatin
Wednesday 01/08/14
10:00a – 5:30p, 9:30p – 11:30p Ph project Ph project model figures for Ajaz. See post Reorganized figure producing code (see Code\Results) Chromatin analysis copying F05 data over to Probox 7 Running analysis of F03 and F04 while copying data … Continue reading
Tuesday 01/07/14
10:00a – 10:15p chromatin data collection Finish O/N STORM on F04. Transfer data from F03 and F04 to Monet start analysis of F03 and F04 data wash out stains for new BX-C regions: F05, G04 (ANTC), F03-F04-F05 combo, F05-A647 F06-A750. … Continue reading
Sunday 01/05/2014
10:30a – 8:00p, 10:00p-11:50p literature continue reading Berlivet et al PLoS Genetics on HoxA ID 19 candidate enhancers by ChIP and 5C 8 of 10 tested LacZ transgenics show limb bud enhancer activity TAD contacts in silent tissue Shh signalling … Continue reading
Saturday 01/04/13
10:00a – 11:55p (sailing canceled do to malfunction of committee boats) coding matlab-storm RunDotFinder “skip existing” option on DaoSTORM I believe is interpreted as resume and in insightM as actually skip. This should be double checked and made clearer. Maybe … Continue reading
Friday 01/03/2014
remotely 10:30a – 11:30a (snowstorm) Winterize bike: 11:30a – 2:00p lab: 2:00p – 9:00p Goals switch road tires for mountains for better stability in the snow Design new primer collection for chromatin library 3. Pick more regions for multi-color experiments: … Continue reading
Monday 12/16/13
9:50a – 1:00a Group meeting 10a – 2p see notes Probe making cy7 dye from Lumipore arrived A750 labeled IDT primers arrived first test gel — try old gel of Bogdan’s (11/18). Ran smeary and fast, primers ran off the … Continue reading
Posted in Summaries
Tagged chromatin, probe making, STORM, STORM analysis
Comments Off on Monday 12/16/13
Sunday 12/15/13
11:00a – 9:00p z-calibration some new ideas for finalizing new z-calibration routine: see if different starting z-position affects curve alignments (isn’t that the whole hypothesis in the first place?) well, all the data comes from the same range, but the … Continue reading
Saturday 12/14/13
11:15a – 2:30p, 6:00p – 11:45p Coding matlab-storm test installation: created new ZhuangLab account Install for Guipeng. DaoSTORM objects to spaces in pathname. adding double quotes ” does not fix. library Prep New function (in Genomics Functions toolbox) function Seq … Continue reading
Posted in Summaries
Tagged cell labeling, chromatin, genomics, probe making, STORM analysis
Comments Off on Saturday 12/14/13
Friday 12/13/13
10:45a – 12:15a Notebook backed up notebook updated wordpress Z-calibration cross correlation doesn’t improve curve alignment visibly — curves are fundamentally a bit different no spatial structure evident Ph Project reviewing simulation results saved as simulationName_2.eps to file Test new … Continue reading
Posted in Summaries
Tagged chromatin, coding, project2, STORM analysis
Comments Off on Friday 12/13/13
Protected: Lab meeting 11/11/13
There is no excerpt because this is a protected post.