Tag Archives: chromatin

Wednesday 01/08/14

10:00a – 5:30p, 9:30p – 11:30p Ph project Ph project model figures for Ajaz. See post Reorganized figure producing code (see Code\Results) Chromatin analysis copying F05 data over to Probox 7 Running analysis of F03 and F04 while copying data … Continue reading

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Tuesday 01/07/14

10:00a – 10:15p chromatin data collection Finish O/N STORM on F04. Transfer data from F03 and F04 to Monet start analysis of F03 and F04 data wash out stains for new BX-C regions: F05, G04 (ANTC), F03-F04-F05 combo, F05-A647 F06-A750. … Continue reading

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Sunday 01/05/2014

10:30a – 8:00p, 10:00p-11:50p literature continue reading Berlivet et al PLoS Genetics on HoxA ID 19 candidate enhancers by ChIP and 5C 8 of 10 tested LacZ transgenics show limb bud enhancer activity TAD contacts in silent tissue Shh signalling … Continue reading

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Saturday 01/04/13

10:00a – 11:55p (sailing canceled do to malfunction of committee boats) coding matlab-storm RunDotFinder “skip existing” option on DaoSTORM I believe is interpreted as resume and in insightM as actually skip. This should be double checked and made clearer. Maybe … Continue reading

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Friday 01/03/2014

remotely 10:30a – 11:30a (snowstorm) Winterize bike: 11:30a – 2:00p lab: 2:00p – 9:00p Goals switch road tires for mountains for better stability in the snow Design new primer collection for chromatin library 3. Pick more regions for multi-color experiments: … Continue reading

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Monday 12/16/13

9:50a – 1:00a Group meeting 10a – 2p see notes Probe making cy7 dye from Lumipore arrived A750 labeled IDT primers arrived first test gel — try old gel of Bogdan’s (11/18). Ran smeary and fast, primers ran off the … Continue reading

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Sunday 12/15/13

11:00a – 9:00p z-calibration some new ideas for finalizing new z-calibration routine: see if different starting z-position affects curve alignments (isn’t that the whole hypothesis in the first place?) well, all the data comes from the same range, but the … Continue reading

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Saturday 12/14/13

11:15a – 2:30p, 6:00p – 11:45p Coding matlab-storm test installation: created new ZhuangLab account Install for Guipeng. DaoSTORM objects to spaces in pathname. adding double quotes ” does not fix. library Prep New function (in Genomics Functions toolbox) function Seq … Continue reading

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Friday 12/13/13

10:45a – 12:15a Notebook backed up notebook updated wordpress Z-calibration cross correlation doesn’t improve curve alignment visibly — curves are fundamentally a bit different no spatial structure evident Ph Project reviewing simulation results saved as simulationName_2.eps to file Test new … Continue reading

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Protected: Lab meeting 11/11/13

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