Tag Archives: fly work

Friday 02/15/13

Posted on February 15, 2013 by admin

9:30 A STORM Finish O/N run crashed hal when computer memory filled up. Need more external harddisks! Should do a test run on if we can save data on the fly from Hal over the network straight to Monet. Still … Continue reading →

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Sunday 02/10/13

Posted on February 10, 2013 by admin

10:30 A – 11:35 P STORM Finish O/N STORM run of Fab7 running new chromatic 3D bead calibration (more positions, slightly narrower z-range) check out Hazen’s new Zee-calibrator — (looks like in progress viewer for data-fit curves). Doesn’t show molecules … Continue reading →

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Thursday 02/07/13

Posted on February 7, 2013 by admin

9:30 AM – 8:30P STORM Finish O/N STORM imaging Pc-488 not bleaching well. Maybe 750 will be better. Fly Work Esc virgins emerging — collecting. some non-Sp (Df/esc[2] — it’s okay). No males yet to score phenotype AM collection 2x … Continue reading →

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Monday 02/04/12

Posted on February 4, 2013 by admin

9:45 A — 6:30 P, 7:30P – 12:50 A Coding Implementing new method to compute z-calibration curves. See my saga of posts on the subject. Here’s the most recent post (with the least descriptive detail). Integrate new z-calibration computation method … Continue reading →

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Sunday 02/03/2012

Posted on February 3, 2013 by admin

11:00 A – 6:30P, 7:30P – 12:30 A STORM Finish O/N STORM run Take bead image z-calibration Take bead images for chromatic correction Copy data to Alistair8 Coding Fixed bugs in file export in STORMfinderBeta Generalize Z-calibration to work with … Continue reading →

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Saturday 02/02/13

Posted on February 2, 2013 by admin

11:30 A – 1P, 1:45P – 12:45 A STORM Overnight run on STORM3 crashed at around 34 of 85 movies — ‘memory error’. send crash report to Hazen. copying data form Alistair9 to Alistair8. Imaging BX-C embryos with H3K27me3. One … Continue reading →

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Friday 01/02/2013

Posted on February 1, 2013 by admin

9:45 A – 7:15P, 8:30P – 10:40 P STORM Record z-calibration curve for STORM3 Finish O/N run of Fab7 on STORM3 Finish O/N run of BX-C embryos on STORM2. Ideal focus plane not matched between regions (this can be custom … Continue reading →

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Tuesday 01/29/13

Posted on January 29, 2013 by admin

10:00 A – 7:20P, 8:45P – 11:45P General As of Today, all IDT oligo orders are to go through Matt, not through HHMI card on file. Cell labeling heating up 2X SSCT wash to 60C in large hybe oven check … Continue reading →

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Monday 01/28/13

Posted on January 28, 2013 by admin

9:45A – 10:00 P Lab meeting (notes) Fly work scored 5 more back-cross lines (no rescues still):  [spreadsheet 0AjSqkxgziU1YdENuMzFjejM0TnNXUEQ1OWdHRW1OY1E 602 202 sheet = 1] Flipped and watered bottle cross of Df/CyO,esc[2] x Sp/esc[6] males. New bottle cross of Df/CyO,esc[2] x Sp/esc[6] males … Continue reading →

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Thursday 01/24/13

Posted on January 24, 2013 by admin

9:30 A – 7:30P STORM * Finish O/N run by 10A. * Run Z-calibration * Copy and delete data!  Remove external hard-drive * Image BX-C + H3K27me3-488 slide (at 4C in PBT).  maybe we should store these in 2x SSCT? … Continue reading →

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