Tag Archives: image analysis

Protected: snail promoter data, images from Mounia

Posted on August 24, 2012 by admin

There is no excerpt because this is a protected post.

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Wednesday 08/22/12

Posted on August 22, 2012 by admin

9:25 A – 7:00P, 8:00P – 11:15P breakdown O/N confocal run of m106 (ThsPr minus proximal enhancer) Continue running dot counting on previous mRNA data process new O/N m106 data.  weird image error after image15.  suspect screw-up with new LSM5 … Continue reading →

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Tuesday 08/21/12

Posted on August 21, 2012 by admin

9:45 A-5:00P, 8:00P-9:45P Write to TRiP about Pc cross to 3x mat Gal4 outcomes (see previous post). Running analysis of Dfd dataset. This dataset seems to run out of memory and freezes matlab.   Should upgrade RAM in Monet. Literature review: … Continue reading →

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Wednesday 08/15/12

Posted on August 15, 2012 by admin

9:45 A – 6:50P, 8:00P – 11:20PM Finish O/N confocal run on slide m105, save data running simulation on full size embryo of snail promoter mutants.  Strong preference for patches.  Earlier activation of repressors supports conditions that create broader snail … Continue reading →

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Tuesday 08/14/12

Posted on August 14, 2012 by admin

9:15 A – 7:00 P, 8:30 P – 11:20 P Finish O/N STORM run of Antp/Tub bead images z-calibration first iteration looks horrible.  May need new few guess parameters.  Attempting multiple iterations. simulating snail patterning with different promoters.  Need substantial … Continue reading →

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Monday 08/13/12

Posted on August 13, 2012 by admin

9:00 A – 6:40P, 8:00P – 10:30P Zeiss780 training (just like 710.  use 10% of previous laser power.  Detector will shut off for protection if too much saturation) Sarah Lab meeting wnt signalsomes and purinsomes Adding mol-list to image functionality … Continue reading →

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Saturday 08-11-12

Posted on August 13, 2012 by admin

12:45 P-6:30P, 8:30P-10:30P Analyzing 08-07-12 bead data for STORM4 a match radius of 7 is required for aligning all the beads correctly due to a dramatic rotational offset in the channels.  This results in 20-30 nm 3D alignment. Substantially stronger … Continue reading →

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Wednesday 08/07/12

Posted on August 8, 2012 by admin

9:50 A – 6:55P STORM4 run on slide 3b still going. tweak to make outside borders as sharp as internal borders in snail code.  Might need to make more repressor cells in simulation. shoot new z-calibration movies in 4 channels … Continue reading →

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Monday 08/06/12

Posted on August 6, 2012 by admin

9:45 A – 5:00P, 10:00P – 12:25A Finish O/N confocal run of slide 3 (Tub + Antp) Copying data from Alistair6 to Alistair5 Lab meeting (Wenqin) Troubleshooting issue with Steve output – not perfect linear relation between microns and pixel … Continue reading →

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Friday 08/03/12

Posted on August 3, 2012 by admin

9:50 A – 8:15 P STORM O/N run still scanning (ran 488 movie 2Hz too long).  Aborted Make new Glox (STORM4 images look dim / not enough photons/switches given brightness of conventional signal).  suspect Glox kept in door might have … Continue reading →

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