Tag Archives: probe making

Wednesday 04/03/13

Posted on April 3, 2013 by admin

9:45 A – 8:00 P, 9:30 P – 12:00 A General Send notes on sog data to Mounia Training on Qbit. Important point: measure samples between 2 and 2.5 minutes after mixing in reaction dye. STORM Analysis Manual analysis of … Continue reading →

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Thursday 02/28/13

Posted on February 28, 2013 by admin

10:20 A -6:45P, 8:15P – 11:55P (workout 8:30-10:20A) Probe Making (1P-6P @ Longwood) emPCR for 100kb yellow, green and black regions (at Wu lab) see new protocol 35-50 ng/uL yield (11 uL total volume) Notebook Looking in to version managing … Continue reading →

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Sunday 01/20/13

Posted on January 20, 2013 by admin

11:00 A – 4:00P, 10:00P-12:00A Coding Frame-by-frame analysis now runs in a platform independent way form the new shared project matlab-storm folder for both DaoSTORM and InsightM Writing new Analysis script (RunDotFinder) — modified, more flexible version of Run_Insight that … Continue reading →

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Saturday 01/19/13

Posted on January 19, 2013 by admin

11:00 A – 7:00 P Snail Rewrite fxn_Figs_y_v_snail to render only figures indicated in list Plotnames and show them in the indicated figure: ff = strfind(Plotnames,’box_CoVratio’); ff = find(1 – cellfun(@isempty, ff)); if ~isempty(ff) figure(Plotnumbers(ff)); clf; … end This facilitates … Continue reading →

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Friday 01/18/13

Posted on January 18, 2013 by admin

10:40A – 8:00P, 10:ooP – 12:15 A (9-10:40 workout session with group) Coding fixed bug in new temp parameter structure. Added autoload functions uiopen in Matlab default launch folder.  The daxviewer STORMfinder is launched when a dax file is loaded. … Continue reading →

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Wednesday 01/16/13

Posted on January 16, 2013 by admin

9:30 A – 7:30 P  Finish comments on counting comparison for ML # Finish revisions Write to MERCK about Probe Making Precipitate and resuspend RNA-FISH DNP probes for tou, inv, en, Antp and Dfd. Make new RNA-Hybe soln to resuspend … Continue reading →

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Tuesday 01/15/13

Posted on January 15, 2013 by admin

10:00 A – 7:00P, 8:30P-11:30P Probe making in vitro transcription note, spreadsheet is interactive, all reactant amounts aside from template DNA (depends on spec’d conc) computed by sheet. Coding working on new code (STORMfinder) to replace interactive selection of dot … Continue reading →

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Saturday 01/12/13

Posted on January 12, 2013 by admin

10:30 A – 8:00P Probe making precipitate probe, resuspend in fresh DNAse free ddH2O snail data analysis run nuc mapping cyto2nucmap for MP08Hz_snaD_2 data modify lsm_viewer to not append embryo numbers when running export all on embryos data without position … Continue reading →

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Friday 01/10/13

Posted on January 11, 2013 by admin

8:30 A – 9:50 P Fly work Flip collection plate 8:30A Fixing embryos 11:30A.  (20 min 8% FA reaction) Flip all polycomb mutants into bottles STORM made new bead slide, 1:25K imaging 2 rounds of beads for 3D fitting K9me2 … Continue reading →

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Thursday 01/10/13

Posted on January 10, 2013 by admin

8:30A – 10:30P Flip collection plate 8:30A Fixing embryos 10:30A.  (20 min 8% FA reaction) O/N confocal runs — 700 at speed 4 still running.  5 not quite as clean. copy data. Make new fly plates (~4 tubes of plates). … Continue reading →

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