Tag Archives: STORM analysis

Wednesday 04/03/13

Posted on April 3, 2013 by admin

9:45 A – 8:00 P, 9:30 P – 12:00 A General Send notes on sog data to Mounia Training on Qbit. Important point: measure samples between 2 and 2.5 minutes after mixing in reaction dye. STORM Analysis Manual analysis of … Continue reading →

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Saturday 03/16/13

Posted on March 16, 2013 by admin

11:00 A – 7:00P Kinetic modeling STORM Analysis Switching is indeed failing on BX-C 2/20 data. Sad we still don’t have this basic data with enough depth yet. S3 cell data shows plenty of blinking out at 58 and 90K … Continue reading →

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Thursday 03/14/13

Posted on March 14, 2013 by admin

10:00 A – 7:40P, 8:30P-10:30P Genomics processing real vanSteensel data now Reading in data and converting to int8 data matrix for data compression.cellfun(@(x) int8(round(20*str2double(x))), data) seems to be running slower than looping the over the columns… Actually need single to … Continue reading →

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Wednesday 03/13/13

Posted on March 13, 2013 by admin

10:00 A – 9:30P, 11:00P – 12:55 A STORM Finish O/N run on STORM2 STORM Analysis Launch Analysis of all fab7 data collected on STORM2 — now running on Cajal in small batches. Finish configuring local repo on TUCK to … Continue reading →

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Wednesday 02/20/13

Posted on February 20, 2013 by admin

10:00 A – 7:15P, 8:30P – 11:15P STORM Samples: BXC cy5 (single primary) and BXC cy5 + 750 secondary BXC-cy5 mounted for imaging 12:00P Realigned optics Taking bead calibration images Working out parameters to do z-scan. Can’t go too far … Continue reading →

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Sunday 02/17/13

Posted on February 17, 2013 by admin

STORM Finish O/N run of BXC + flank try2 (3D this time). Did not lose TIRF. Did not overfill drive or crash computer (4GB left on hard-drive, 400 written). Copying to new Alistair10. (will move to the new Alistair9 when … Continue reading →

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Saturday 02/16/13

Posted on February 16, 2013 by admin

10:30 A – 8:30P, 10:00P – 11:55P STORM Analysis DaoSTORM analysis still running on Fab7-A647 Pc-750 Read write steps from USB connected hard-drive very slow when this analysis is running. (I’m not sure how much slower the analysis goes to … Continue reading →

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Wednesday 02/06/13

Posted on February 6, 2013 by admin

9:00 A – 8:30P, 9:30P-11:00P Mulitplex STORM covergalss shattered en route from HMS to cambridge, extracting fragments of slides, successfully ID Bx-C Pc. stained BX-C with Pc-488 (new ab-cam anti-rb). Stained additional Pc labeled slide with Pc-750. (1 hr + … Continue reading →

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Thursday 01/24/13

Posted on January 24, 2013 by admin

9:30 A – 7:30P STORM * Finish O/N run by 10A. * Run Z-calibration * Copy and delete data!  Remove external hard-drive * Image BX-C + H3K27me3-488 slide (at 4C in PBT).  maybe we should store these in 2x SSCT? … Continue reading →

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Monday 01/14/13

Posted on January 14, 2013 by admin

9:30 A – 7:30P snail imaging copy data from confocal (55 GB file).  Run O/N at speed 7 using GASP detector projecting data from false start.  Delete lsm file (mostly blank, and the critical data has been copied into a … Continue reading →

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