Author Archives: admin

Tuesday 01/14/14

Posted on January 14, 2014 by admin

9:45a – 12:15a Goals Today Rewrite ColorIntervalJumpGap.m Function to allow different gaps for different chromatin types. Select new Red regions Chromatin Project analysis Designing some new regions: notes Writing up summary of chromatin regions thus far: see protected notes. Let’s … Continue reading →

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chromatin library organization and design

Posted on January 14, 2014 by admin

Introduction / Motivation Current library 3 design is still missing some key regions we would like to investigate very soon. For example there is no concerted attempt to bring red regions back into the fold. Preliminary observations of red regions … Continue reading →

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Protected: Survey summary, Library 2 chromatin data

Posted on January 14, 2014 by admin

There is no excerpt because this is a protected post.

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Monday 01/13/14

Posted on January 13, 2014 by admin

9:15a – 12:15a Plans for week probe making troubleshooting probe smears. Give Jeff some library material. make new probes (F01, F02, F07) + control + some of Jeff’s probes test different RNase inhibitors? Order Library 3 trim probe list (remove … Continue reading →

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Meeting XZ

Posted on January 13, 2014 by admin

Requests / timeline from XZ complete broad survey of regions before diving too deep on models hopefully one or two parameters can explain scaling between different regions naturally unsatisfying if a different / unrelated model is invoked for each scaling … Continue reading →

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journal club 01/13/14: tech apps

Posted on January 13, 2014 by admin

Causes and effects of N-terminal codon bias (Goodman, Church and Kosuri). Science Influential approach Combine 2 Techs oligo synthesis on a programmable microarray – diverse These authors combine with next gen sequencing – 10-200 million unique sequences in a pool … Continue reading →

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Protected: lab meeting 01/13/14

Posted on January 13, 2014 by admin

There is no excerpt because this is a protected post.

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Sunday 01/12/14

Posted on January 12, 2014 by admin

4:00p – 5:20p, 5:50p – 10:30p chromatin data analysis F03, F04, F05, and BX-C data does current spit dax work correctly on 512×256 images? Or are half the frames skipped? or something worse? seems to work just fine. Ran bead … Continue reading →

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Friday, 01/10/14

Posted on January 10, 2014 by admin

9:45a – 6:15p STORM imaging – multi-color chromatin O/N imaging of F5 F6 lost focus very early in night. O/N imaging buffer also did not survive well Re-imaging F11 F12 new freshly made Glox 2.5 uL BME + 5 uL … Continue reading →

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Thursday 01/09/14

Posted on January 9, 2014 by admin

9:50a – 5:30p, 10:00p – 12:15a Goals file reimbursement paperwork for post-doc visit check to Colenso for ski trip help Sebastian design probes chromatin project Run probe design for new chromatin regions selected to test off-diagonal HiC contacts Too many … Continue reading →

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