Category Archives: Fly Work

Thursday 06/14/12

Posted on June 14, 2012 by admin

10:00A – 7:20P, 8:40 P – 11:30P Start running analysis on MT data from yesterday Flip fly collection cages, 10:10 A 4 new bottles of Pc-GFP and Yw each.  5/28 Pc-GFP bottles ready to transfer to collection cage, should give … Continue reading →

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Friday 05/04/12

Posted on May 4, 2012 by admin

12:00 P – 9:30 P 8:30-12P volleyball practice update probe & plasmid library update antibody labels collect virgins set up shRNA crosses x Pc shRNA2 and Psc shRNA 1 in collection cages PCR for in vitro transcription rxn to make … Continue reading →

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Sunday 04/22/12

Posted on April 22, 2012 by admin

10:30 A -5:00P Fly work Flipped all Gal4-Bcd3’UTR lines into new bottles (8).  Moved to 25C.  Flip again in 2-3 days, start virgin collection in 10 days. New cages of MP09, yw, and Pc-GFP. Disposed all bottles of MP09 and … Continue reading →

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Sunday 04/01/12

Posted on April 1, 2012 by admin

8:50 A — 11:30P Finish O/N confocal of MP08. For Zeiss710: a 1.7 um offset from center, with 8 um difference between top plane and imaging plane for the first sample only (with other samples all set to top plane) … Continue reading →

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Wednesday 02/29/12

Posted on February 29, 2012 by admin

9:45 A — 11:00P Washing out O/N 4C antibodies from new protein stains Flip Fly stocks Flip vials and enter new GFP lines into fly stocks. Start expanding Gal4 bcd-3′ UTR (to try crossing into PcG RNAi lines.  will need … Continue reading →

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Friday 02/03/12

Posted on February 3, 2012 by admin

10:10 A – 9:30 P Flip collection cages, 10:15 A Prep new coverslips (2 racks) Flip collection cages 12:15 P clear sna[D] flies for virgin collection Flip fly stocks Flip MP09 and 4x snail flies transfer laid embryos to make … Continue reading →

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Tuesday 11/22/11

Posted on November 22, 2011 by admin

10:00 A – 8:00P, 9:00P-10:00P Finishing annotation of revised CheckDotUpDown code to do 3D dotfinding. Running test analysis of MP06 data. New method for finding unique spheres is easier to read but much much slower, need to find some solution … Continue reading →

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Monday 07/18/11

Posted on July 18, 2011 by admin

8:20A Projecting 2.8het sna sim counting data Analyzing 2.8het sna sim data Projecting MP12 Hz data Analyzing MP12 Hz data Confocal MP05Hz repeat (start warmup 2:15). Finish flipping stocks except APT eye screening 3 new bottles of AP-toll Lab meeting … Continue reading →

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Thursday 07/14/11

Posted on July 14, 2011 by admin

8:10 A – 4:45P Start flipping fly stocks (1-50 flipped) Mean wt sna levels /slide vary between 150 and 250. Project MP10_b data.  1 duplicate and 1 part bleached image.  8 good embryos. brk on Y and brk deletion lines … Continue reading →

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Protected: Feedback/followup from lab meeting

Posted on July 5, 2011 by admin

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