Monthly Archives: April 2011

Imaging bursting?

Posted on April 12, 2011 by admin

Imaging transcriptional bursting directly? .  

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Tuesday 04/12/11

Posted on April 12, 2011 by admin

10:10 A – 7:03 P, 8:10 P – 11:00 P Water Perry’s fly crosses Flip collection cages: 10:45 A Fix embryos: MP09 Hz, MP02 Hz, MP10 Hz; sna[18]/CyoZ (1.5-4.5 hrs) Flip AP-Twi, AP-Twi2 and AP-toll lines Imaging transcriptional bursts (see … Continue reading →

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Monday 04/11/11

Posted on April 12, 2011 by admin

10:00 A – 7:15P, 8:00P – 12:15A revising JF research proposal with dad’s feedback. Confocal too much drift, only 1 image.  needs immediate, 1.5 hr warm up.  extremely frustrating.  1.5 hr imaging of separate slide before finding samples not sufficient. … Continue reading →

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Protected: adding enhancers draft

Posted on April 11, 2011 by admin

There is no excerpt because this is a protected post.

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Sunday 04/10/11

Posted on April 10, 2011 by admin

4:ooP – 7:15P, 8:30P – 11:00P Finish ON confocal data disk full.  Drive shuffling, back up 2010 stuff and before onto Fantom Drive 1 Tb external.  Delete from primary data hard-disk to free up space. Confocal work: can’t find vnd, … Continue reading →

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Saturday 04/09/11

Posted on April 10, 2011 by admin

10:45 A -8:10 P, 9:00P — 11:45 P Finish extended ON confocal at 12:50P, 11 embryos completed (50 sections, 16.17um, 330 nm spacing). Tighten up JF research proposal, send to Carl and dad for edits. Working on collaboration proposal for … Continue reading →

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to image

Posted on April 9, 2011 by admin

Slides to image still: 04/06/10: C33, C55, untouched 22C and 30C 0-4 hr slides.  Need cc13 embryos 01/07/10: 3x snail in vnd[D38] 01/07/10: E(spl) x vnd[D38], E(spl); E(spl)/HbZ

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Mapping MS draft: Overlapping elements improve pattern

Posted on April 9, 2011 by admin

Figures Kr kni multiple enhancers (proximal, distal, both) sog temperature series, proximal, distal, both sog conservation (for temperature speculation) sog total intensity variation hb LacZ ? model for promoter limited multiplicative action

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Friday 04/08/11

Posted on April 8, 2011 by admin

10:35 A – 8:40P, 9:40P – 11:00P Working on JF research proposal Accept offer for Banff stochasticity workshop, Sept 11-17. cede afternoon confocal time to JB Confocal MP01, 11 embryos set up.

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Thursday 04/07/11

Posted on April 7, 2011 by admin

9:40 A — 5:00 P, 10:40P – 12:05 A reset fly incubators to normal temperature and humidity after oven bake. proof-check Ecology paper.  Sent back to GW. working on JF research proposal and collaboration proposal fix embryos (MP09 Hz and … Continue reading →

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