Monthly Archives: December 2011

7:45 A – 12:02 A Finish O/N run of MP08 confocal, 9 embryos scanned, 10 frames into position 10, nice mesodermal view of embryo.  next user cancelled, could have finished 11 embryos, check email first. wash out primary antibodies on … Continue reading →

10:30 A – 11:15 P fix MP08 and wt embryos, 1.5-4 hrs MP09 embryos now in resin embedding O/N new antibody stains (bcd, hb;  ftz, nuclear lamin-B, H3K4me3, H3K27me3) in 15 min F fixed embryos.  2 hr primary at RT, … Continue reading →

9:40 A – Embryo collection MP08, sna[D].  2 hr collections and 2 hour aging before fixing: 10-12, 12-2, 2-4, 4-6:30, wt collection: 2-4, 2.5-4.5 hr, 4.5-6.5 hr, 1 -4.5 write matlab script to convert dax files to averaged pngs (for … Continue reading →

11:00A – 12:15 A in situs day 3 STORM imaging: Invitrogen Po-Po family dye 5 and dye 6 do not seem to survive embedding process — see outline of nuclei nicely but no DNA staining. H3K27ac sections show punctate spots … Continue reading →

11:30 A – 9:00P, 11:00P – 12:00A in situs day 2 aliquot Heparin and ssDNA to avoid freeze-thaw cycles in situ notes: embryos in vial 1 turn yellow, fixation difference? 30 min sodium ethoxide treatment for histone-3 slide. STORM imaging … Continue reading →

11:45 A – 8:45 P arrive back from Berkeley in situs Day 1: see Embryo_staining log. Make new fly plates, large and small. new fly cages, large wt and small MP08 write to Reinitz

9:00 A – 10:30 P STORM imaging:  for testing of DNA dyes should prep more cc14 embryos — clearer separation of nucleated and non nucleated regions. Very little fluorescence signal detectable. cut new sections H3 (confirmed on confocal), H3K27ac, dye … Continue reading →

7:40 A – 1:2:50 A break down O/N confocal run of sna[D] embryos Reviewing counting results of s142_sna (wildtype, imaged Saturday night through Sunday). change Enhancer_modeling to mRNA_counting (note: the git repositories for Image_Analysis and Shadow_Enhancers are published urls (Perry … Continue reading →