Monthly Archives: January 2012

Saturday 01-14-12

Posted on January 14, 2012 by admin

11:00A – 7:50 P No engrailed stain No evidence that short fix, antibody label, then in situ, actually labels mRNA efficiently. No hb-mRNA detected. attempted imaging of en-dig (cut yesterday) 50 min etching removed all cells, only bits of yolk … Continue reading →

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Friday 01/13/12

Posted on January 13, 2012 by admin

10:10 A -8:00P Start etching K4 Flip MP08 cage (10:40A) New MP08 cage cut eng-dig sections 3 – 4:30 cut new Ftz, eng labeled embryos 4:30 -6p, section’s of previously trimmed block do not hold together well. cut selection of … Continue reading →

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hunchback and bcd antibody testing

Posted on January 12, 2012 by admin

some quick checks looking at dynamically refinement of hb gradient. Hb (green) and Bcd (red) double stained (early cc13?)         zoom , showing subnuclear localization patterns.           showing very gradual hb transition   … Continue reading →

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Protected: Wednesday 01/11/12

Posted on January 12, 2012 by admin

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Tuesday 01/10/12

Posted on January 10, 2012 by admin

11:30 A – 7:00P writing fellowship apps etching Antp slide. Make new Glox solution. imaging Antp sections on STORM 2: no detectable Antp or ubx transcripts.  647 channel (H3K27) looks backgroundry? etching en-flu (previous slide had sections too near the … Continue reading →

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