Monthly Archives: February 2012

STORM session: (hox slide 1)

Posted on February 16, 2012 by admin

STORM imaging 2/16/12 Laser calibration at output: 750 100 mW, 647 200 mW 561 100 mW (200 at laser) 488 200 mW 405 1.3 mW (max why low?) substantial resin still on slide S7 etched yesterday for 30 min. etched … Continue reading →

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Thursday 02/16/2012

Posted on February 16, 2012 by admin

9:40 A — 12:10 A collect virgins O/N STORM run did not finish on schedule (68 of 112 images done) contact Wu lab about choice DNA FISH regions install GTX580 card. install latest NVIDIA drivers received Rad21 antibodies in 50% … Continue reading →

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Wednesday 02/15/12

Posted on February 15, 2012 by admin

9:45 A — 11:00P Etching Dm0-1 slides (25-30 min) for STORM.  (Slide 69 in records) Flip fly cage, 10A Washing out primary antibody in PcG stained embryos in situs for PcG stained embryos: hox and en locus (see records) organized … Continue reading →

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Protected: PcG in mitosis summary

Posted on February 15, 2012 by admin

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Tuesday 2/14/12

Posted on February 14, 2012 by admin

10:00A  — 12:20 A  Flip fly cage, 10:00A confocal imaging of H2 (multi histone stains, slide 70) embryos to cut. Mark region imaged by confocal using dissecting scope in CBI. confocal images of S6, S7, H3, sections marked, thin-sectioned onto slides. … Continue reading →

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Monday 02/13/12

Posted on February 13, 2012 by admin

10:00A -7P, 10P-12:20A Visiting postdoc talk (Bryan). 10A-1P Flip fly cage 1P. Some STORM stacks didn’t process in batchinsightm.  Some issues still in running code, tweaked code and re-running missing data sets: S45, S44ubx, Pc, S45_13i. Working on image registration … Continue reading →

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Protected: H3K4me3 by confocal and STORM

Posted on February 12, 2012 by admin

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Sunday 02/12/12

Posted on February 12, 2012 by admin

9:45 A — 5:20P, 8:30P — 11:50P Finish STORM O/N of S45 (13-introns) setup STORM run of Pc Flip fly cage (10:50P) Flip fly cage (1:50P) Hal holds control of 647 laser AOTF against labview. Fixed. Analyzing STORM data by … Continue reading →

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Hox summary

Posted on February 12, 2012 by admin
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Saturday 02/11/12

Posted on February 11, 2012 by admin

11:00A — 11:45 P Collect hard disk and slide from O/N STORM run analyzing confocal data from S44 (antp/ubx/H3K4). new confocal images: Pc, Su(Z)12, S44 making new antibodies (with KE) see records: 12P remove samples from oven (13 hours) let … Continue reading →

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