Monthly Archives: March 2012

Tuesday 03/13/12

Posted on March 13, 2012 by admin

11:00A – 11:30P O/N run of GPU code on Hb data aborted due to Matlab figure/windows waiting hang-up. Working on DR app request Smith et al Lidke Lab GPU astigmatism fit Received GPU astigmatism fit code New cages of MP09 … Continue reading →

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Tuesday 03/06/11

Posted on March 12, 2012 by admin

10:15 A – 8:00P, 9:30P –  Ordered 2 new RAIDed external hard-drives for next experiments Running analysis on Hb dataset.  1.3.-2 million localizations per movie in 750 channel, each movie maxes out a processor — throttle at 7 movies / … Continue reading →

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Fly Meeting 2012

Posted on March 7, 2012 by admin

Plenary Session I. Thummel (Utah) – Regulation of energy metabolism in drosophila Metabolic tissues and pathways conserved through flies. (e.g. insulin/glucagon balance) Will develop obsiety and diabetes (including insulin resistance, cardiovascular disorders etc) Transcriptional control by nuclear receptors: bind DNA … Continue reading →

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Monday 03/05/12

Posted on March 5, 2012 by admin

11:00 A -8:30 P, 9:30 P – 11:00P Running analysis on Psc-K27 data. Test view of H3-Cy3B not look promising.  Despite very promising conventional images. Illumination on Cy3B channel was not even, top of image substantially dimmer.  Need to check … Continue reading →

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Sunday 03/04/12

Posted on March 4, 2012 by admin

10:40 A – 10:30 P Working on color registration for S2 data Laying out motivation slides for Fly Meeting presentation Working on fly meeting slides Fixed STORM2: 405 laser not working required Hal reboot. Reinstalling square aperture required bead slide … Continue reading →

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Saturday 03/03/12

Posted on March 3, 2012 by admin

11:00A – 7:00 P Overnight run of Psc H3K27 aborted after ~23 sections (235 Gb) . Moving files from Alistair2 (G:\) to Alistair3 (K:\) to mobilize Alistair2 for data collection tonight. Can copy files over local intranet quickly from G … Continue reading →

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Friday 03/02/12

Posted on March 2, 2012 by admin

10:05 A -1:00 A Flip new PcG shRNA lines. Enter into stocks. Screen MP12 rescue crosses.  set up 6 new crosses, 3 with bright eyes might be homozygous MP12. 1 vial with straight wings tossed and 1 vial with Sp … Continue reading →

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Recent STORM image gallery

Posted on March 2, 2012 by admin
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Compare 2D and 3D spot finding results

Posted on March 2, 2012 by admin

2D in green, 3D in red (H3-647).   Z images are collapsed down into single plane.  It looks like brightness differences are substantial, better contrast total photons from 2D imaging (might have expected reverse, 3D apparently doesn’t compensate for photon … Continue reading →

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Thursday 03/01/12

Posted on March 1, 2012 by admin

9:50 A – 12:10 A Transfer PcG and Hb/Bcd samples to ethanol for embedding Running Insight3 analysis on single section images from Tuesday / Wednesday STORM session. Discussion with Graham and Colenso: Try switching to BME for Drosophila PcG shRNA … Continue reading →

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