Monthly Archives: April 2012

Friday 03/20/12

Posted on April 20, 2012 by admin

11:55 A – 8:00P, 9:30 – 11:30P (volleyball 8:50A-11:50A) need missing reference letter and electronic submission for ASC app! running STORM analysis on 04/05/12 En-tou-Epc-H3 data. Rerunning chromatic alignment on 04/14/12 en-Epc conventional data working on slides for group-meeting presentation

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Thursday 04/19/12

Posted on April 19, 2012 by admin

9:50 A – 8:00P, 9:30 – 10:30P Working on group meeting presentation background slides figure out and implement gaussian dot fitting in matlab use precise fitting to locate molecules in conventional images of En-Epc-H3 stained embryos, superimpose chromatically corrected images. … Continue reading →

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Wednesday 04/18/12

Posted on April 18, 2012 by admin

9:45 A -8:00P, 9:30P-11:00P Running alignment on all E(Pc)-en-H3 data Used H3 4-color experiment beads.  should use correct slide beads. Running bead analysis for all E(Pc)-en-H3 data. Create new fxns for bead runs.

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Tuesday 04/17/12

Posted on April 17, 2012 by admin

9:40 A – 8:00P, 9:40P-11:45P Finish O/N storm run Running H3 4-color STORM data processing on Cajal.  Z-calibration analysis with autoZ script gives crazy results.  Using previous parameter files for analysis. Zcal error created from system grabing data files instead … Continue reading →

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Monday 04/16/12

Posted on April 16, 2012 by admin

9:50 A – 12:20 A Lab meeting Yari Lab discussion on STORM scopes: should be used all night, should upgrade STORM3 so more users can do their experiments — need 488 line and 647 line. Wenqin’s Thesis defense Journal club … Continue reading →

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Sunday 04/15/12

Posted on April 15, 2012 by admin

4:30 P – 11:00P STORM imaging. HP1-750 slide 1 not fully etched, only some nuclei switch. 1hr 15 min etching. STORM imaging Hp1-750 slide 2 over-etched, 750 switching great, 647 dye almost all lost, very weak by conventional and few … Continue reading →

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Saturday 03/14/12

Posted on April 14, 2012 by admin

11:00A – 7:00P, 9:00P – 11:50P O/N run overwrote day run of en H3.  have to make a new subfolder! The O/N run was using one of the powerfiles from the afternoon run. When this got overwritten the powerfile got … Continue reading →

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Friday 04/13/12

Posted on April 13, 2012 by admin

9:20 A – 5:00P, 8:30 P – 12:00A miniprep Taf and Tub samples Register with GeneWiz and send samples for sequencing STORM imaging of en-Epc-H3 continues, focus lock lost early this morning when moving to top of new strip.  Pause … Continue reading →

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Thursday 04/12/12

Posted on April 12, 2012 by admin

9:20 A – 11:50 P Both large and small plates look good. large plates a bit sparse but still good colonies to chose from. Benny Shilo seminar coding automated iterations of z-fitting in matlab. oligo-paint- good staining!  high DNA background … Continue reading →

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Wednesday 04/11/12

Posted on April 11, 2012 by admin

10:00A – 11:30P coating 5o mm coverslips put coverslips in metal, vertical mount holder.  Don’t stay balanced in ceramic holder.  Need to alternate positions or the coverslips stick. Do coating in 400 mL in 1000 mL beaker (not 300 mL … Continue reading →

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