Monthly Archives: November 2012

issues with non-blinking weak spots

Posted on November 15, 2012 by admin

The very bright, round foci clearly do not correspond to objects in the conventional image and are probably all artificats from non-switching foci. These dots start off at moderate single molecule brightness (can’t eliminate them just using higher threshold cutoff … Continue reading →

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Wednesday 11/14/12

Posted on November 14, 2012 by admin

9:00A – 7:30P, 8:15P – 11:15P Prepping gel (low on Na Borax, and TAE making up new TAE) Test gel: YW postive control AbdB looks great.  YW negative control sim[D] is clean.  Improvised gel extractions not looking good. Try sim[D] … Continue reading →

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Tuesday 11/13/12

Posted on November 13, 2012 by admin

9:15 A – 6:50P, 8:00-9:30P STORM analysis 750 still running, min height probably too low.  Raised defaults for next time. Prep for genotype screening Order primers for testing MP10 MP05 differences (seems like labeled MP10 lacks expression). DNA extraction using … Continue reading →

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Monday 11/12/12

Posted on November 12, 2012 by admin

9:45 A – 7:30 P, 8:45P – 10:00P Troubleshooting issues with bead warping wrote short translation correction to (1D polywarp) to correct the gross aberrations introduced by the STORM4 quadview. Still seems to be a shift.  Might be due to … Continue reading →

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Sunday 11/11/12

Posted on November 11, 2012 by admin

10:15 A – 5:00 P, 8:20 – 9:00P working on BJ review figures write BJ review captions review NSF proposal Start Analysis of Inv data  Run bead analysis Need to fix crazy quadview offset from STORM 4 with a translation … Continue reading →

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Testing small oligopaint probes

Posted on November 10, 2012 by admin

First small test probe Reagents The primer  has a 5′ TYE665 dye. Both primers are at 200 µM (200 pmoles/µl).  template was at 10 ng/µl. A look at the genomic region Zoom in on region   Question for Ting /Brian … Continue reading →

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Saturday 11/10/12

Posted on November 10, 2012 by admin

10:45 A – 8:00P Snail project stuff Collect virgin Pr/Tm3Z, Sp/CyOZ, and TM2/TM6 (males + virgins) Order primers for screening E(spl) and sim[D].  Also for en and inv introns DNA FISH probe design Wrote new matlab script for concatinating text … Continue reading →

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Friday 11/09/12

Posted on November 9, 2012 by admin

9:30 A – 8:30P Making double mutants Collect virgin TM2/TM6 flies Clear Pr/TM3,hbZ and Sp/CyOZ for virgin collection to remake double balanced 2,3 flies. TM6b = AntpHue1 . TM2 = Ubx130esemc2 . E(spl) and sim[D](7646) both have TM6b, Tb.  Should be lethal with TM6. … Continue reading →

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Protected: Notes from meeting with Welcome Bender

Posted on November 9, 2012 by admin

There is no excerpt because this is a protected post.

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Troubleshooting new hard-drive install

Posted on November 8, 2012 by admin

Windows doesn’t like talking to new disk drives, and repeatedly connecting new drives seems to create a problem.  Especially when the drives are formatted for Mac. Symptom: The newly plugged in drive does not appear. In start menu, search for … Continue reading →

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