Monthly Archives: December 2012

Saturday 12/15/12

Posted on December 15, 2012 by admin

10:30 A – 7:00P Working on presentation Check split cells. S2R+ ~80% confluent, should be able to split tonight. Kc167 < 50% attached Genome browser Tubigen version of Galaxy not talking to UCSC.  Spent several hours troubleshooting workflow only to … Continue reading →

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Research Planning 12/14/12

Posted on December 15, 2012 by admin

Use antibody accessability as a proxy for PolII accessability. Compare levels of staining of H3K27me3 in intact vs. mechanically spread nuclei.  (First we need to see if H3K27me3 even remains associated in spread nuclei.  Try spreading nuclei using the ‘shearing’ … Continue reading →

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Friday 12/14/12

Posted on December 14, 2012 by admin

10:15 A – 8:00 P, 9:00P – 11:30P Probe making LC library arrived today IDT primers arrived Cell culture diluted recovering S2R+ culture and Kc160 cells (prior to splitting). Move back to 28C incubator Testing temperature in 28C incubator. S2 … Continue reading →

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Thursday 12/13/12

Posted on December 13, 2012 by admin

10:15 A -7:00P, 8:00P – 9:45P, STORM imaging TIRF controller crashed.  Will not revive. After hours of various attempts and help from several lab members, Graham finds in manual that power cycling the controller fixes the problem.  Unplug the black … Continue reading →

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Wednesday 12/12/12

Posted on December 12, 2012 by admin

10:30 A – 7:30P, Data analysis Analyzing Hp1a centromere data Labeling density still somewhat low, centromere certainly not ‘coated’ with Hp1a by imaging. Might be an antibody accessability thing.  Should compare with bio-labeled centromere probe.  I remember when I last … Continue reading →

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Tuesday 12/11/12

Posted on December 11, 2012 by admin

10:10 A Check PCRs (in blocks 3A (sim screening) and 3B (Espl screening) better way to do this: complementation test back to original sim[D] and Espl[D] lines.  Just need to expand those lines back to bottles, will need ~100 vials … Continue reading →

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Monday 12/10/12

Posted on December 10, 2012 by admin

9:50 A -7:40P, 8:40P – 12:30 A yari lab meeting De-noising: use conventional image as low pass filter to remove non-blinking background Use conventional alignment for drift correction, translate all STORM movies to match multi-color conventional image. Use field of … Continue reading →

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Sunday 12/09/12

Posted on December 9, 2012 by admin

10:20 A – 7:30P, 9:30-11:00P STORM 750 1600 mA = 18 mW 1.45 mW 405 200 mW set 647 Made new Glox 750 not switching well.  Not sure if it’s just low labeling (405 looked okay but not strong). 647 … Continue reading →

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Saturday 12/08/12

Posted on December 8, 2012 by admin

11:00 A – 7:30P, 8:45P – 10:00P Cell staining label with m-HP1a -405,750 and rb-H3K27me3 -488 (top) or rb-H3K27ac -488 (bottom row) Polytene cell staining label with m-HP1a-Cy3b and rb H3K4me3 -488 or rb H3K27me3-488. check staining on confocal some … Continue reading →

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Friday, 12/07/12

Posted on December 7, 2012 by admin

9:45 A – 8:20 P Stop O/N confocal Prep for ultra sectioning Discussion with W. Bender Graded AbdB expression probably produced by combinatoral enhancer action (multiple enhancers leading to the stronger pattern) early enhancers are somewhat segment like.   Not … Continue reading →

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