Monthly Archives: January 2013

Friday 01/18/13

Posted on January 18, 2013 by admin

10:40A – 8:00P, 10:ooP – 12:15 A (9-10:40 workout session with group) Coding fixed bug in new temp parameter structure. Added autoload functions uiopen in Matlab default launch folder.  The daxviewer STORMfinder is launched when a dax file is loaded. … Continue reading →

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Thursday 01/17/13

Posted on January 17, 2013 by admin

10:30 A – 7:00P, 8:30P – 9:30P (8:30 – 10:30 workout session) Probe making combine, precipitate and pellet en-647 oligoPAINT probe # Wash, combine, and resupend probe-pellet, # Prep for digestion reaction Cell culture (500 kbp probe test 2) After … Continue reading →

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Research goals

Posted on January 16, 2013 by admin

Oligo Paints Week of 01/15/13: synthesis en-Cy5 probe (by my hands in Zhuang lab and by Brian’s trainee at HMS) and of BX-C locus (by Brian). Monday-Tuesday (01/21-22/13), test in situs of en and BX-locus Tuesday: section Drosophila yw embryos … Continue reading →

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Wednesday 01/16/13

Posted on January 16, 2013 by admin

9:30 A – 7:30 P  Finish comments on counting comparison for ML # Finish revisions Write to MERCK about Probe Making Precipitate and resuspend RNA-FISH DNP probes for tou, inv, en, Antp and Dfd. Make new RNA-Hybe soln to resuspend … Continue reading →

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RNA in situ hybe buffers

Posted on January 16, 2013 by admin

Hybe Solution 50% Formamide (Amresco, ultrapure) 5x SSC 100 ug/mL salmon sperm DNA 50 ug/mL heparin 0.1% Tween-20   Reagent For 250 mL stock Formamide 125 mL 20x SSC 62.5 mL Heparin 10 mg/mL lab aliquot 1.25 mL 10% Tween-20 … Continue reading →

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RNA in situ hybridization

Posted on January 16, 2013 by admin

 Day 1 Xylene clearing ~2 hrs Rock embryos in 1:1 MeOH:EtOH 5 min (Waste -> organic waste bottle) Rinse 2x with EtOH Rock in EtOH 5 min Remove all but 100 uL EtOH, Add 900 uL Xylenes 1.0 hr (embryos should … Continue reading →

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Tuesday 01/15/13

Posted on January 15, 2013 by admin

10:00 A – 7:00P, 8:30P-11:30P Probe making in vitro transcription note, spreadsheet is interactive, all reactant amounts aside from template DNA (depends on spec’d conc) computed by sheet. Coding working on new code (STORMfinder) to replace interactive selection of dot … Continue reading →

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synthesis of RNA in situ probes

Posted on January 15, 2013 by admin

Set up in vitro transcription reaction as follows: Reagent Volume 5x transcription buffer (ProMega): 4ul 10x hapten (Dig. Bio DNP) U-NTP mix 2ul 100mM DTT 1ul RNase inhibitor (RNasin) (50 μ/μl) 2ul linearized DNA (~250 ng if 0.2μg/μl) 1ul (vary … Continue reading →

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Monday 01/14/13

Posted on January 14, 2013 by admin

9:30 A – 7:30P snail imaging copy data from confocal (55 GB file).  Run O/N at speed 7 using GASP detector projecting data from false start.  Delete lsm file (mostly blank, and the critical data has been copied into a … Continue reading →

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Protected: Steven lab meeting, 01/14/13

Posted on January 14, 2013 by admin

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