Monthly Archives: February 2014

Thursday 02/21/14

5:30p – 8:00p, 9:00p – 10:30p (snowshoeing). Random Walk Polymers Quick reference with the scaling of the self-avoiding RWP (exponent = 3/(2+d), where d is the spatial dimension). Thus 0.6 for 3D SAW polymer More interesting stuff http://arxiv.org/pdf/0711.3679v1.pdf Pivot + … Continue reading

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Protected: XZ meeting notes

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Wednesday 02/19/14

9:45a – 11:45p Goals Today Final prep for meeting with Xioawei add slides showing density STORM analysis launching drift bead analysis for F05 / F06 images and Ph-iFISH data launching split-dax for all un-analyzed datasets on RAID2 and Pro8. running … Continue reading

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Protected: Draft outline for chromatin paper

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Tuesday 02/18/14

9:45a – 7:00p, 9:00p – 12:20a STORM Imaging G123 (sample 1 from last staining). Very, very high nuclear background (and strong signal). surprising given the concentration used, but maybe should try 1/10th concentration. STORM analysis Cajal crashed, all jobs got … Continue reading

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STORM Users planning meeting

STORM2 Max Laser Powers 488 laser head (100%): 580 mW 488 before expander: 275 mW 488 microscope backport: 180 mW 561 at head (2100mW in GUI): HIGH 561 laser at beam expander: 1500 mW 561 laser at backport 900 mW … Continue reading

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Monday 02/17/14

9:30a – 12:45p Lab organization measure laser powers on STORM4 STORM users meeting — see summary sent in email. Ph project Paper to do We need to make the sup. fig1. showing no pri-antibody controls. added the flag in S2 … Continue reading

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Saturday 02/15/14

5:50p – 11:30p (Sailing day) Deep Sequencing analysis continuing analysis of sequencing data, looking at F11 probes. Windows navigator refuses to browse to Data folder. Might have to do with the current read write operations from this folder. Still pathetic. … Continue reading

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Sunday 02/16/14

1:30p – 7:00p, 10:30p – 12:10a Chromatin Project Library3 Prep (renewed) Revised gene list Code updating Fixed minor bug in BatchLaunchOligoArray: ProcessTimeOut for blastall was in seconds but for java was in minutes. Fixed bugs in GetLocusSenseSeq — had extended … Continue reading

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Friday 02/14/14

10:00a – 7:15p Deep sequencing analysis Effect of Seq Prep on Abundance Prepping samples for sequencing this time at least doesn’t seem to introduce large distortion in abundance. Would have been good to sequence these libraries much deeper though, average … Continue reading

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