Monthly Archives: July 2015

Thursday 07/23/15

Posted on July 23, 2015 by admin

12:30 pm – 7:00 pm (sick) RNAi experiments Primer issues diagnosing problems with primers for qPCR writing code for primer mapping organizing a better data structure to keep track of all the RNAi targets, their qPCR primer sets, their RNAi … Continue reading →

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Wednesday 07/22/15

Posted on July 22, 2015 by admin

9:30 am – 8:45 pm Psc deletion picked up psc deletion cells from Sonny, along with media (also gifted a second aliquot of the Pc-antibody), now frozen. also gifted ~200 mL of media ready mixed. cells were last plated 7/12 … Continue reading →

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Tuesday 07/21/15

Posted on July 21, 2015 by admin

10:15 am – 8:00 pm RNAi experiments Quantifying knockdown efficiencies by qPCR cDNA PPPES (Pc, Ph-p, ph-d, Esc, Scm) rep 1 PPPES (Pc, Ph-p, ph-d, Esc, Scm) rep 2 Pc v1 Pc v2 Pc, Esc, Scm, Esc Suz12 Scm SCE … Continue reading →

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Monday 07/20/15

Posted on July 20, 2015 by admin

9:00 am – 8:00 pm Goals prep new RNAi treated cells for staining Plate, fix and lyse current RNAi treated samples RNA isolation prep cDNA production qPCR reactions (?) [tomorrow] Set up synthesis reactions for new RNAi RNAi experiments new … Continue reading →

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Protected: lab meeting: 07/17/15

Posted on July 17, 2015 by admin

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Thursday 07/16/15

Posted on July 16, 2015 by admin

9:10 am – 1:05 am MERFISH data analysis Lots of little coding changes Changes to LoadMERFISHdax Handling collections of info files cell arrays of structures are hard to index these should just be multi-element structures rewriting all info file stacks … Continue reading →

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Wednesday 07/15/15

Posted on July 15, 2015 by admin

9:10 am – 7:00 pm, 8:50pm – 1:00 am MERFISH Analyzing MERFISH data, (9:10 am – 1:00 pm) see post MERFISH meeting (1:00 – 2:30 pm) Jeff’s talk (4:00 pm – 5:15 pm) rerunning analysis with nearest third of pixel … Continue reading →

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Protected: MERFISH 07/15/15

Posted on July 15, 2015 by admin

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Tuesday 07/14/15

Posted on July 14, 2015 by admin

10:00 am – 8:00 pm Goals make cDNA from RNAi samples from last week qPCR of RNAi samples from last week set up more RNAi for next week make more dsRNA for RNAi reply to Hao, comments on essay analyze … Continue reading →

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Protected: SDB 2015 day 4

Posted on July 12, 2015 by admin

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