Tag Archives: coding

Friday 02/08/13

Posted on February 9, 2013 by admin

11:00 A – 8:30P, 9:30P-11:00P (remote) Fly work collect virgins non-Sp CyO males of Esc not obviously extra sex combs (only 1 male so far). Need to check bristly number and frequency expected (see Vivek’s paper). new bottles of MTDs … Continue reading →

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Saturday 02/09/13

Posted on February 9, 2013 by admin

11:00 A – 8:30P 9:30 – 10:15P (remote) (blizzard) STORM Finish O/N run of BXC+Pc take new bead calibration movies setup imaging of FAb7+Pc current beadslide has substantial tilt — maybe we replace it sometime Fly work collect virgin mat … Continue reading →

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Thursday 02/07/13

Posted on February 7, 2013 by admin

9:30 AM – 8:30P STORM Finish O/N STORM imaging Pc-488 not bleaching well. Maybe 750 will be better. Fly Work Esc virgins emerging — collecting. some non-Sp (Df/esc[2] — it’s okay). No males yet to score phenotype AM collection 2x … Continue reading →

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Monday 02/04/12

Posted on February 4, 2013 by admin

9:45 A — 6:30 P, 7:30P – 12:50 A Coding Implementing new method to compute z-calibration curves. See my saga of posts on the subject. Here’s the most recent post (with the least descriptive detail). Integrate new z-calibration computation method … Continue reading →

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z calibration part 3

Posted on February 4, 2013 by admin

Maximal alignment achieved through simulated annealing. Basically we have a whole bunch of short fragments of the longer, true z-curve, and we don’t know their z-offset displacement. So we jiggle them around and find the parts of them that match. … Continue reading →

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Z-calibration part 2

Posted on February 4, 2013 by admin

Curves fit the following polynomial: $latex w_x=\sqrt{w_{x0} \sqrt{B_xX^4+A_xX^3+X^2+1}}$ where $latex X= (z-g_x)/(z_{rx})$ In general we will require $latex gx$ to be negative $latex gy$ to be positive. $latex Ax$ and $latex Bx$ are correction factors and should be small. See … Continue reading →

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Z-calibration

Posted on February 3, 2013 by admin

Same set of molecules at a collection of different heights Challenges Stage tilted: all molecules in a given frame DO NOT have the same wx wy distortion. Stage started position uncertain — might not be mid-range. Stage z-drift during movie … Continue reading →

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Sunday 02/03/2012

Posted on February 3, 2013 by admin

11:00 A – 6:30P, 7:30P – 12:30 A STORM Finish O/N STORM run Take bead image z-calibration Take bead images for chromatic correction Copy data to Alistair8 Coding Fixed bugs in file export in STORMfinderBeta Generalize Z-calibration to work with … Continue reading →

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Coding Goals 02/02/13

Posted on February 3, 2013 by admin

Short Term Goals DaoSTORM Clean up calls for DaoSTORM in STORMfinder to use simplier DaoSTORM installation. (Complete 02/02/13) Test run DaoSTORM Merge all active projects in Matlab_STORM into Beta Move latest version of Insight3 and DaoSTORM in matlab-storm. (completed 02/02/13) … Continue reading →

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Saturday 02/02/13

Posted on February 2, 2013 by admin

11:30 A – 1P, 1:45P – 12:45 A STORM Overnight run on STORM3 crashed at around 34 of 85 movies — ‘memory error’. send crash report to Hazen. copying data form Alistair9 to Alistair8. Imaging BX-C embryos with H3K27me3. One … Continue reading →

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