Tag Archives: STORM

Friday 06/15/12

Posted on June 19, 2012 by admin

10:50 A – 7:2oP Collection cages flipped, 10:30A. Troubleshooting but in Dave-program with Hazen on STORM2.  frames randomly dropped from end of movie.  some movies randomly dropped. System is not repeating the movie drop.  Does repeatedly drop the last frame. … Continue reading →

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Wednesday 06/13/12

Posted on June 14, 2012 by admin

10:00 A-7:00P, 8:00P -11:00P STORM4 training with Yari STORM4 imaging microtubules and 3D beads.  Stage drifts rapidly (including between switching runs) unless joystick is set just right (slight tilt to left). Day 3 labeling embryos.  Joint directly labeled primaries K27me3, … Continue reading →

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Monday 06/11/12

Posted on June 11, 2012 by admin

10:00 A – 7:00P, 8:30P-11:30P Hao lab meeting in situs day 1 (by KE): protein structure between hox targets Fixing AOTF problem with STORM2.  Between beam shape and AOTF, no usable light is passed. AOTF removed from table. analyze probe … Continue reading →

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Saturday 09/06/12

Posted on June 9, 2012 by admin

11:45 A – 7:15P, 8:30P – 10:30P Staining microtubules in 4 colors for imaging control (with help form Graham) Finish O/N STORM run of Pc-GFP with before and after 3D beads and z-calibration movies Finish staining no primary controls of … Continue reading →

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Friday 06/08/12

Posted on June 8, 2012 by admin

10:00 -8:00P, 9:00P -11:20P Flip fly cages Finish clean up O/N STORM run.  Coopying files from Alistair5 to Alistair4. move in situ probe-test slides into boxes.  (Move archive boxes into 4C bin to make more space in 4C drawer?) 11:00 … Continue reading →

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Thursday 06/07/12

Posted on June 7, 2012 by admin

10:20 A – 6:50P, 8:30P – 11:20P in situs day 3 with KE. 17 slides mounted to test tomorrow [slideshow 0AjSqkxgziU1YdG90a29UaU5ua1FLUXZ5LTFmM1pMSlE 600 300 sheet=7] Prep STORM slide of Su(Z)12-750, K27-647 STORM imaging of Pc-GFP slide Beads arrays in 3 focal planes … Continue reading →

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Saturday 05/05/12

Posted on May 5, 2012 by admin

11:00A -5:30P Talk title for Poland wash out primary antibodies Nanodrop gel-extraction samples STORM cryo-section samples: considerable out of focus light makes for hazy looking conventional images, though nuclei and membranes are clearly distinguishable in the respective channels. 647 STORM … Continue reading →

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Wednesday 05/02/12

Posted on May 2, 2012 by admin

10:25 A – 8:50P Wash out O/N H3K27me3 on Pc-GFP labeling embryos Etch H4 (H3K27, K4): 1h 25 min sequences back from GeneWiz: Fab8B +/-, inside msa ncRNA (-) Fab8A, +/- inside msa ncRNA (-) AbdB-2B +/+ antisense. use T7 … Continue reading →

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Sunday 04/29/2012

Posted on April 29, 2012 by admin

4:00 P -9:30 P STORM analysis of Pc-GFP: Insight froze on 647 channel at ~1.5 million molecules : error program tried to terminate incorrectly. min height on all parameter files somehow got stuck at 750 levels (30).  Reset manually.  stem … Continue reading →

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Friday 04/27/12

Posted on April 27, 2012 by admin

10:20A – 5:10P, 9:00P – 11:00P Etch hb-y(i), Bcd sample STORM hb-y(i), Bcd sample remember to turn 642 laser power back up! miniprep colonies: AbdB-1A,B AbdB-3A,B, Dfd-2A,B, 5E-A,B.  all over 100 ng/uL. send 500 ng for sequencing to GeneWiz. move ligation … Continue reading →

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