Tag Archives: STORM

Monday 04/16/12

Posted on April 16, 2012 by admin

9:50 A – 12:20 A Lab meeting Yari Lab discussion on STORM scopes: should be used all night, should upgrade STORM3 so more users can do their experiments — need 488 line and 647 line. Wenqin’s Thesis defense Journal club … Continue reading →

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Sunday 04/15/12

Posted on April 15, 2012 by admin

4:30 P – 11:00P STORM imaging. HP1-750 slide 1 not fully etched, only some nuclei switch. 1hr 15 min etching. STORM imaging Hp1-750 slide 2 over-etched, 750 switching great, 647 dye almost all lost, very weak by conventional and few … Continue reading →

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Saturday 03/14/12

Posted on April 14, 2012 by admin

11:00A – 7:00P, 9:00P – 11:50P O/N run overwrote day run of en H3.  have to make a new subfolder! The O/N run was using one of the powerfiles from the afternoon run. When this got overwritten the powerfile got … Continue reading →

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Friday 04/13/12

Posted on April 13, 2012 by admin

9:20 A – 5:00P, 8:30 P – 12:00A miniprep Taf and Tub samples Register with GeneWiz and send samples for sequencing STORM imaging of en-Epc-H3 continues, focus lock lost early this morning when moving to top of new strip.  Pause … Continue reading →

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Thursday 04/12/12

Posted on April 12, 2012 by admin

9:20 A – 11:50 P Both large and small plates look good. large plates a bit sparse but still good colonies to chose from. Benny Shilo seminar coding automated iterations of z-fitting in matlab. oligo-paint- good staining!  high DNA background … Continue reading →

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Monday 04/09/12

Posted on April 9, 2012 by admin

10:00 A – 10:30 P Lab meeting (Doory) Fix alignment of 750 laser with Colenso’s help Freeze Bcd-GFP and eve-GFP bottles. NuGru presentation (Andi) Working on snail manuscript supplemental figure captions Download ModEncode data for H3K4me3 and H3K27ac Etch and … Continue reading →

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Saturday 04/07/12

Posted on April 7, 2012 by admin

10:00 A – 7:15 P Confocal: embedded en-E(Pc)-tou-H3 sample does indeed have good intronic staining.  Not many 330 nm sections happen to contain the locus.  Plenty but still a minority of cells are clearly transcribing E(Pc).    So most sections … Continue reading →

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Thursday 04/05/12

Posted on April 5, 2012 by admin

10:00 A — 8:30 P Take GFP embryos out of 4C, incubating at RT heating up 2x SSCT for hot wash mounted oligo-paint embryos before and after washes (with WGA-488 for post-wash embryos).  Remaining embryos at 4C Cut sections of … Continue reading →

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Friday 03/23/12

Posted on March 23, 2012 by admin

10:45 A – 12:30 A O/N confocal 10 um is a little too much buffer between first and last, several embryos didn’t make it all the way through.  Try again tonight. Set up new O/N confocal run 6 um difference … Continue reading →

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Wednesday 03/21/12

Posted on March 21, 2012 by admin

12:00P – 9:30P (9A-12P lab volleyball practice) in situs day 2 protein stains wash out primaries, post-fix, add secondaries. started new page, see record: Kill old bottles of MP09, yw.  Need to set up new bottles STORM imaging of 3Mb … Continue reading →

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