Tag Archives: fly work

Monday 07/30/12

Posted on July 30, 2012 by admin

9:50 A – 5:00 P, 9:30P – 10:45p Jiang lab meeting difficulty connecting to notebook 710 confocal all sessions canceled, new 780 being installed this week.  Requested training on ELYRA which also has a 710 (and maybe a programable stage?) … Continue reading →

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Wednesday 07/18/12

Posted on July 18, 2012 by admin

10:10A – 9:30P working on new SDB talk (kinetics, no bistability) Issue with text animation.  Just on windows system. working on bead fitting.  06-25-beads (100nm) fit pretty well, see all 5 regions in most channels (vis have 4 clear and … Continue reading →

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Wednesday 07/11/12

Posted on July 12, 2012 by admin

10:10 A – 5:ooP check antibody locations with KE in situs day 3 Confocal: tub and Ant-C genes look good.  Preliminary data suggests loci mostly paired in older embryos but not condensed — substantial separation distance. Ubx and AbdB have … Continue reading →

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Monday 06/25/12

Posted on June 25, 2012 by admin

9:45 A – 7:00P, 9:30P – 11:45P Need new collection cages FISH + K27 on O/N embryos day 1 Flipped 3x Mat Gal4 cages. Running Z-calibration analysis on Suz647 data (with Quadview) Collecting new z-cal bead data in 512 and … Continue reading →

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Thursday 06/21/12

Posted on June 21, 2012 by admin

2:00P — 8:30P Calibrating new bead imaging: 1:20 then 1:500 540 beads + 1:500 Q-dots-705.  Too dense. Dilute a further 1:500.  Q dots good density.  Blinky, excited best by 405 or 488.  Look good in 647, not really visible in 750 … Continue reading →

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Wednesday 06/20/12

Posted on June 20, 2012 by admin

10:50 A – 6:30P ordered 0.04 um Fluospheres (540 + 715).  large fluospheres give unusable 3D maps No 715 Fluospheres, ordered 20-nm 705 quantum dots Fixed error in Z-cal convergence.  Modified convergence measure to use both x and y data. … Continue reading →

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Thursday 06/14/12

Posted on June 14, 2012 by admin

10:00A – 7:20P, 8:40 P – 11:30P Start running analysis on MT data from yesterday Flip fly collection cages, 10:10 A 4 new bottles of Pc-GFP and Yw each.  5/28 Pc-GFP bottles ready to transfer to collection cage, should give … Continue reading →

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Tuesday 06/05/12

Posted on June 5, 2012 by admin

10:00A – 8:00P in situs day 1 with KE: new large cage of Pc-GFP and Yw Flip 3x Mat-Gal4 into new bottles.  Moved to 25C. troubleshooting chromatic warp issues in new vector storm code.

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Monday 05/28/12

Posted on May 28, 2012 by admin

10:40 A – 7:00P, 8:45P – 11:45 P Flip fly bottles: Pc-GFP.  Now growing at 25C Flip into single bottles for bottle stock: Pc-shRNA1 and 2, Psc-shRNA1,  Bcd-GFP, eve-GFP, en-GFP.  Pc-shRNA lines strongly female gender biased. Primary stocks at 18C look … Continue reading →

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Monday 05/14/12

Posted on May 14, 2012 by admin

10:00A – 8:30P, 9:30-10:30P Eileen Group meeting invitation to SDB to chair postdoc session and speak in regulatory mechanisms section NuGru presentation: Swr1 structural analysis (inserts H2AZ flanking nucleosome depleted regions), for example to protect promoter. Finished 3D bead warping … Continue reading →

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