Tag Archives: STORM

Monday 08/26/13

Posted on August 26, 2013 by admin

9:45a – 6:30p STORM finish O/N STORM run of AbdA cells Cell culture changed media on 3 new Kc-cell flasks plated media on polyL coated coverglass. cells somewhat sparse, let’s give them some time to grow before fixing. Data clean-up: … Continue reading →

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Friday 08/23/13

Posted on August 23, 2013 by admin

10:15a – 8:10p, 8:50p-11:05p BX-C tiling Finish O/N STORM of Ubx spots start 65C 50% formamide rinse out of probe (30 min) Hot wash of newly labeled Ubx (orginally AbdA): 60C 2x SSCT Image Ubx. Correspondence of cells and correspondence … Continue reading →

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Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

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Wednesday 08/21/13

Posted on August 21, 2013 by admin

9:45a – 11:30p cell staining heating buffer. wrong buffer, no formamide in 60C hot wash. Did I use the formamide buffer yesterday? I recall heating a 50mL tube and I don’t think I have 2x SSCT in a 50mL tube… … Continue reading →

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Saturday 08/17/13

Posted on August 17, 2013 by admin

10:30a – 10:30p Helping Hao align FRET Wrote SplitQVdax.m SplitDaxFRET_130817 Note: splitting large dax files is slow and memory intensive. Even 2000 frames takes a few gigs in the memory map approach. (10:30-2:30). Will need to help get setup with … Continue reading →

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Thursday 07/25/13

Posted on July 25, 2013 by admin

STORM break down O/N run of R2bxcD STORM analysis run all bxc data dot-fitting (que up on Tuck in small batches, Cajal not talking) run all bxc mosaic file conversions (que up on Cajal in linear batches) Embryo staining incubating … Continue reading →

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Monday 07/22/13

Posted on July 22, 2013 by admin

9:30 A – 7:00 P, 8:30 P – 11:50 P STORM Finish O/N set up new STORM, coverslip 7. high background, must by cy5-primary. not too much switching, maybe single labeled cy5 primary. Responsive to 405 but only at substantial … Continue reading →

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Thursday 07/18/13

Posted on July 18, 2013 by admin

9:30 A – 7:45 P, 8:30 P – 12:00 A expanding primer sets looking into quaternary cyclic codes to generate primers with appropriate distance There is literature on this, but it is not obvious from the proofs and coding theory … Continue reading →

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Thursday 07/11/13

Posted on July 11, 2013 by admin

10:00 A – 7:00P, 8:30P-10:00P STORM Finish O/N run of PhM-flag-488, Ph-647 take bead images transfer data to ProRAID Set up analysis to run on Cajal Embryo in situs wash out probes check staining: All embryos washed off! 1 tiny … Continue reading →

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Wednesday 07/10/13

Posted on July 10, 2013 by admin

10:00 A – 7:40 P, 8:30 – 10:00 P General laboratory safety training refresher check HoxD server jobs: still heavily backed up check STORM analysis / drift correction: Y complete. Others still running. update notebook entries from yesterday. Review working … Continue reading →

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