Tag Archives: confocal

Sunday 08/26/12

Posted on August 26, 2012 by admin

3:30 PM -:715P, 8:30P – 10:30P STORM run lost focus.  Haven’t analyzed yet at which position / how many movies lost. Setup new collection of positions.  All pretty old embryos in this batch, K27me3-647 stain doesn’t look great, channel has … Continue reading →

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Thursday 08/23/12

Posted on August 23, 2012 by admin

9:50 A-7:00P O/N confocal run not completed.  Increased speed to 6, running until 2p. Trying to run daoSTORM3d.  path files all screwed up, won’t work. Fixed path files (hazen has a script which can be called prior to execution). To … Continue reading →

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Tuesday 08/21/12

Posted on August 21, 2012 by admin

9:45 A-5:00P, 8:00P-9:45P Write to TRiP about Pc cross to 3x mat Gal4 outcomes (see previous post). Running analysis of Dfd dataset. This dataset seems to run out of memory and freezes matlab.   Should upgrade RAM in Monet. Literature review: … Continue reading →

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Friday 08/17/12

Posted on August 17, 2012 by admin

10:00 A  – 8:45P, 10:00P-11:45P Etching slide 1 from 6/25 in situs, 1 Dfd-dig, tub-bio.  25 min etch. Mostly over-etched.  setting up for STORM imaging of remaining sections:. more snail simulations. Approach 1, see if kicking in a twist activation of … Continue reading →

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Thursday 08/02/12

Posted on August 2, 2012 by admin

9:50A — 6:20, 8:20p – 11:55P Confocal O/N — not enough sections / .17 spacing too close to make it through nuclear layer.  trashed image. STORM O/N — Hal froze on movie 48.  Sent crash report to Hazen.  Shut down … Continue reading →

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Wednesday 08/01/2012

Posted on August 1, 2012 by admin

10:10 A – 7:30P, 8:20P – 10:45P Flip collection cages. Meeting with Ting and Xiaowei. KE miniprep cg5… cg10… and bxd samples.  lab colonies did not grow in liquid culture?  Sent samples for sequencing. STORM of Pc6_cntrl Label new dk-rb-750 … Continue reading →

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Saturday 07/28/12

Posted on July 28, 2012 by admin

10:30 A -2:00P, 4:30P-7:30P, 9:50P-11:00P Finish O/N confocal of m197.  Imaging plane too high, lower sections of most embryos not fully imaged.  Set up rescan. ~10:30A. working on parsing genomic data to test changes in H3K27me3 occupancy in A8 using … Continue reading →

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Friday 07/27/12

Posted on July 27, 2012 by admin

9:40 A – 7:40, 9:00P – 12:30A Finished confocal run, 8.5 embryos finished at speed 5 by 10A (run started at 10P). Previous user canceled 710 time (again).  Need to check before starting or stopping a run. Over-etched first K27 … Continue reading →

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Tuesday 07/24/12

Posted on July 24, 2012 by admin

10:00A – 12:30A Revising snail kinetics paper for NSMB Revised Supp Fig 4, revised kinetics paragraph for multicopy.  Recomputed approach to saturation regime controlled by intrinsic kinetics of Pol II. confocal imaging of sample 1 from Mounia KE repeating PCR for new … Continue reading →

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Tuesday 07/17/12

Posted on July 17, 2012 by admin

10:10 A – 7:00P, 8:20 P – 11:10P working on presentation for SDB teaching primer design for KE: new primers for: bxd, lab, #scr, CG5520, CG3995, CG10326, CG10324.  Ordered. KE confocal of last weeks embryo staining in vitro transcription reactions … Continue reading →

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