Tag Archives: images

Thursday 08/29/13

Posted on August 29, 2013 by admin

8:15a – 7:00p Multi-stain analysis try crosslinking 415 beads to fresh, unfixed cells initial beads somewhat clumped and extremely bright, especially under high 405 illumination (or minimal weak 488 illumination). Beads do successfully blead into 647 channel at moderate brightness … Continue reading →

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PCR

Posted on August 28, 2013 by admin

Amplifying G1-8 using T7 primers for each sublib 18.5 ddH2O 5 uL 5uM common 1 uL library (cut to .5, at 1uL I can only do each lib once) 25 uL Phusion master master mix (9x) 166.5 uL ddH2O 45 … Continue reading →

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Thursday 08/22/13

Posted on August 22, 2013 by admin

10:10a – 7:00p, 8:50p – 12:10a STORM & staining BX-C tiles AbdA labeled coverslip (S2 cells) found cross-hatching imaging additional cells record chromatic beads will restain AbdA-stained coverslip with BX-C Ubx labeled coverslip (S2 cells) found cross-hatch (much more evident … Continue reading →

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Friday 07/19/13

Posted on July 19, 2013 by admin

9:50 A – 5:30 P Banff finish draft schedule upload draft schedule to website finish activities sheet send participant email list, activities sheet, draft schedule, and notes about filming talks and requesting titles to Andrew. Planning with Jeff for next … Continue reading →

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Thursday 07/18/13

Posted on July 18, 2013 by admin

9:30 A – 7:45 P, 8:30 P – 12:00 A expanding primer sets looking into quaternary cyclic codes to generate primers with appropriate distance There is literature on this, but it is not obvious from the proofs and coding theory … Continue reading →

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Protected: Ph Data Pc Psc effects

Posted on July 8, 2013 by admin

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Monday 07/01/13

Posted on July 1, 2013 by admin

9:45 A – 8:00 P 9:00 P – 12:00 A STORM O/N STORM lost focus early in night :(. Reset focus, got a few more cells during 4.5 hour lab meeting. Replaced buffer on chiseled PhF slide. Setting up to … Continue reading →

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Protected: Ph update

Posted on June 24, 2013 by admin

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Protected: project 2

Posted on June 5, 2013 by admin

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Tuesday 05/21/13

Posted on May 21, 2013 by admin

8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading →

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