Tag Archives: Library2

Thursday 10/10/13

Posted on October 10, 2013 by admin

10:00a – 8:30p, 10:00p – 12:00a Goals finish abstract for Alvaro work on slides for Stowers STORM with Bogdan finish F1, start coverslip 4 STORM data processing with Bogdan Oligo Prep G3-G5 P1A405, P2Cy3, P4Cy3 samples Hybridizations for next small … Continue reading →

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Wednesday 10/09/13

Posted on October 10, 2013 by admin

9:00a – 12:35a Goals work on Presentation for Stowers STORM Ran out of disk space, crashed O/N Run lost focus anyway immediately after position 0. Probably not enough oil. Possibly positions were chosen too far apart. Need to explain these … Continue reading →

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Monday 10/07/13

Posted on October 7, 2013 by admin

9:45a – 8:00p, 1:00a – 2:00a General Group meeting cancelled XZ meeting cancelled Ph modeling Discuss analysis methods with Jeff Moment comparisons. nmin / cv^2 way different than 1, definetely not exponentional (also obvious by visual inspection, median 4-7, mean … Continue reading →

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Wednesday 10/02/13

Posted on October 2, 2013 by admin

10:00a – 11:30p Goals today repeat plate PCR, work on technique, set up for sequencing STORM pipeline, G10 425kb black. start new small black. work on Banff Report – NO PROGRESS work on slides for XZ meeting – [Full time … Continue reading →

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Tuesday 10/01/13

Posted on October 1, 2013 by admin

9:45a-4:15p, 7:45p – 11:40p Goals Today order new cy3 and 405 primers with new secondary binding sites order new cy5 and cy7 (amine?) secondary oligos for multi-color experiments Work on Banff report sign up for qPCR training at the Bauer … Continue reading →

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Friday 09/27/13

Posted on September 27, 2013 by admin

9:00a – 4:30p Multiday Goals Write letter to Ting and company Design D mel. gene library complete ANT-C stains (G1-G5) (need G1 probe to complete). Probe Making PCR cleanups: Large Regions D11 100 kb percicentric Green D12 385 kb Yellow … Continue reading →

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Thursday 9/26/13

Posted on September 26, 2013 by admin

10:00a – 10:40p Goals Laundry email Ting & Co. draft letter on progress on goals on chromatin Analyze existing data for new spots: density, puncta etc. scale factors? (density vs. length or something?) Computer splitdax aborted in night when ProRAID … Continue reading →

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Wednesday 09/25/13

Posted on September 25, 2013 by admin

9:30a – 11:15p STORM stop O/N STORM start copying data to Monet from O/N start spot-finding analysis on yesterday’s O/N splitdax F6 data. G2 data also not analyzed: set up DaoSTORM analysis on Cajal G2 bead data looks sparse — … Continue reading →

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Tuesday 09/24/13

Posted on September 24, 2013 by admin

11:00a-4:30p, 8:00p-11:00p Goals: Cell culture plate cells on Poly-Lysine coverglass (with Bogdan ?) passage remaining cells (with Bogdan ?) Prep 9/2 plate and new plate for DNA FISH. Staining O/N primary staining of Pc and anti-flag. Probe making RNA cleanup … Continue reading →

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Monday 09/23/13

Posted on September 23, 2013 by admin

9:30a – 7:30p, 9:00p – 11:50p Meetings 10a, lab meeting 11:45a, meeting with Ting and Fred Need to send letter to team. Cell in situs Wash out primary probes STORM E6 (tertiary approach still) — very weak spots visible in … Continue reading →

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