Tag Archives: Ph

Ph project manuscript to do

Posted on March 12, 2014 by admin

Add Pc to figure 1 (all Pc, not just Ph associated Pc) Follow same layout of 3 panels, confocal, STORM, stats. Fig 6 diameter as a function of Ph-ML concentration. Modeling supplement: detailed description and chemical equations Fig 3. fix … Continue reading →

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Monday 03/03/14

Posted on March 3, 2014 by admin

9:30a – 12:05a Project 2 Out of disk space on Tuck and temp drive. analyzing data, team meeting, writing new code, and more stuff: see project post. The little things passage Kc cells, replace cell media make new STORM imaging … Continue reading →

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Monday 02/17/14

Posted on February 17, 2014 by admin

9:30a – 12:45p Lab organization measure laser powers on STORM4 STORM users meeting — see summary sent in email. Ph project Paper to do We need to make the sup. fig1. showing no pri-antibody controls. added the flag in S2 … Continue reading →

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Friday 02/14/14

Posted on February 14, 2014 by admin

10:00a – 7:15p Deep sequencing analysis Effect of Seq Prep on Abundance Prepping samples for sequencing this time at least doesn’t seem to introduce large distortion in abundance. Would have been good to sequence these libraries much deeper though, average … Continue reading →

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Monday 02/10/14

Posted on February 10, 2014 by admin

9:30a – 11:50p Meetings Group meeting (see notes) Journal club team project meeting Literature interesting perspective: http://www.sciencemag.org/content/343/6171/596.full Pauli’s paper: discovering new developmental signalling peptide (Science) Chromatin Data analysis Analyzed G01-G02 up to image 23 start (re-)analyzing F03. F03 data analyzed … Continue reading →

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Saturday 02/08/14

Posted on February 8, 2014 by admin

10:30a – 4:45p, 10:00p-11:50p Chromatin Deep sequencing of libraries finish gel extraction aborted last night due to mini-centrifuge failure. Data analysis Analyze new ANTP data issue: need to fix integrated drift correction, have some weird errors interpolation was not correct … Continue reading →

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Friday 02/07/14

Posted on February 7, 2014 by admin

10:00a – 11:30p PH project Meetings Finalize slides discussion with XZ Add effect on cluster count instead of effect on cluster size. Updates working on figure for the effects on cluster count added cluster counts vs. cluster diameter some further … Continue reading →

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Thursday 02/06/2014

Posted on February 6, 2014 by admin

10:00a – 12:50a Ph Project Meeting with Ajaz, discuss paper revisions. continue preparing slides for discussion with XZ. Meeting notes (4p – 7:30p) To image by Confocal (top priority) Endogenous Ph Endogenous Pc Endogenous Psc (may not work) Ph-Flag Ph-M-Flag … Continue reading →

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Wednesday 02/05/2014

Posted on February 5, 2014 by admin

10:00a – 6:00p, 9:30p-11:50p Ph Project Data processing start fitting PhM flag + Psc data on Cajal start fitting PhWt flag + Psc data on Cajal Presentation prep Seminar on making slides (see notes) start making slides for PH project … Continue reading →

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Tuesday 02/04/2014

Posted on February 4, 2014 by admin

11:00a – 8:00p, 9:30p – 10:30p Ph project Manuscript writing working on model section finished description of first model (on-rate or off-rate limited) finished writing description of second model, ~3:30p, (chromatin availability limited) working on methods section wrote description of … Continue reading →

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