Tag Archives: presentation

Tuesday 11/06/12

Posted on November 6, 2012 by admin

9:15 A – 9:00P, 10:ooP-11:00P (vote, 8:45A) Washing out probe from on slide DFISH.  hot wash at 60C -forgot for 2hrs (oops!) stain with Hoechst and WGA-488 Working on presentation for Francis group meeting STORM characterizing embryo background (see images) … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Tuesday 11/06/12

Monday 11/05/12

Posted on November 5, 2012 by admin

9:30A -10:00P Overview of today Flip sim[D] plate (9:30A) Colenso Lab meeting Age sim[D] plate and 3 MTD x SCM plates Fix sim[D] and SCM embryos.  Should be ready for in situs Working on Francis lab group meeting presentation / … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Monday 11/05/12

Wednesday 10/10/12

Posted on October 10, 2012 by admin

10:00 A – 8:00P, 9:00P – 11:15P Collect virgins Working on presentation for meeting with Xiaowei (see notes below on data processing and analysis). Meeting with XZ.  Notes here. Processing STORM data 8/25/12 AbdA, no 750 movies analyzed yet in … Continue reading →

Posted in Research Planning, Summaries | Tagged , , | Comments Off on Wednesday 10/10/12

Tuesday 10/09/12

Posted on October 9, 2012 by admin

10:00 A – 7:20P, 8:30P – 11:30P Fly Work collect virgin MTDs.  (progeny of MTD x TRIP -> collection cage.   These embryos should lay infertile offspring / transformed embryos).  Let’s do Psc; MTD first, see if structure of K27me3 … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Tuesday 10/09/12

Monday 10/01/12

Posted on October 1, 2012 by admin

9:30 A – 7:00P, 8:30P – 11:00P Finished integrating STORMcuts and STORMrender.  Moved primary function call to separate directory (routines). Jeff M lab meeting. Send info on metabolic labeling/DTA (dynamic transcriptome analysis) ordered pyranose oxidase (use at 3U/mL instead of … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Monday 10/01/12

Wednesday 07/18/12

Posted on July 18, 2012 by admin

10:10A – 9:30P working on new SDB talk (kinetics, no bistability) Issue with text animation.  Just on windows system. working on bead fitting.  06-25-beads (100nm) fit pretty well, see all 5 regions in most channels (vis have 4 clear and … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 07/18/12

Tuesday 07/17/12

Posted on July 17, 2012 by admin

10:10 A – 7:00P, 8:20 P – 11:10P working on presentation for SDB teaching primer design for KE: new primers for: bxd, lab, #scr, CG5520, CG3995, CG10326, CG10324.  Ordered. KE confocal of last weeks embryo staining in vitro transcription reactions … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 07/17/12

Monday 07/16/12

Posted on July 16, 2012 by admin

9:40A – 5:00P Etching s6: 5E-dig, AbdB-bio.  K27-647 looks good on Turnkey.  Etched 22 min in second use of sodium ethoxide (at 22C for 2 weeks, now dark brown). Review knife care with KE.  (Trimming knife was badly chipped during … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Monday 07/16/12

Thursday 07/12/12

Posted on July 12, 2012 by admin

10:15 A — 8:20 P, 9:20P – 12:00A Finish writing draft cover letter for kinetics paper.  Send to ML. in situs embedding (day 4) KE. coat new coverslips KE. update code repos (need to do this more often). working on … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Thursday 07/12/12

Thursday 06/14/12

Posted on June 14, 2012 by admin

10:00A – 7:20P, 8:40 P – 11:30P Start running analysis on MT data from yesterday Flip fly collection cages, 10:10 A 4 new bottles of Pc-GFP and Yw each.  5/28 Pc-GFP bottles ready to transfer to collection cage, should give … Continue reading →

Posted in Fly Work, Summaries | Tagged , | Comments Off on Thursday 06/14/12