Tag Archives: probe making

Friday 09/27/13

Posted on September 27, 2013 by admin

9:00a – 4:30p Multiday Goals Write letter to Ting and company Design D mel. gene library complete ANT-C stains (G1-G5) (need G1 probe to complete). Probe Making PCR cleanups: Large Regions D11 100 kb percicentric Green D12 385 kb Yellow … Continue reading →

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Thursday 9/26/13

Posted on September 26, 2013 by admin

10:00a – 10:40p Goals Laundry email Ting & Co. draft letter on progress on goals on chromatin Analyze existing data for new spots: density, puncta etc. scale factors? (density vs. length or something?) Computer splitdax aborted in night when ProRAID … Continue reading →

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Wednesday 09/25/13

Posted on September 25, 2013 by admin

9:30a – 11:15p STORM stop O/N STORM start copying data to Monet from O/N start spot-finding analysis on yesterday’s O/N splitdax F6 data. G2 data also not analyzed: set up DaoSTORM analysis on Cajal G2 bead data looks sparse — … Continue reading →

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Tuesday 09/24/13

Posted on September 24, 2013 by admin

11:00a-4:30p, 8:00p-11:00p Goals: Cell culture plate cells on Poly-Lysine coverglass (with Bogdan ?) passage remaining cells (with Bogdan ?) Prep 9/2 plate and new plate for DNA FISH. Staining O/N primary staining of Pc and anti-flag. Probe making RNA cleanup … Continue reading →

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Monday 09/23/13

Posted on September 23, 2013 by admin

9:30a – 7:30p, 9:00p – 11:50p Meetings 10a, lab meeting 11:45a, meeting with Ting and Fred Need to send letter to team. Cell in situs Wash out primary probes STORM E6 (tertiary approach still) — very weak spots visible in … Continue reading →

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Wednesday 09/18/13

Posted on September 18, 2013 by admin

9:15a – 11:30p Writing working on BIRS workshop report (9:15a- 12p) Probe making / training with Bogdan test PCR from yesterday common primer concentration was a bit low gel didn’t fully dissolve, might have affected gel running: Repeat PCRs. Repeat … Continue reading →

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Sunday 09/08/13

Posted on September 8, 2013 by admin

Goals Run gel to test RT reactions from plate-based T7 (complete) second proof read of review article, prepare to submit (not complete) STORM imaging (complete) Review started rereading and editing replies to reviewers. Results E6, E7, E10, F6 with P1 … Continue reading →

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Friday 09/06/13

Posted on September 6, 2013 by admin

8:50a – 5:10p, 8:30p – 10:40p Other stuff write to landlady about move in return keys for 2nd floor Coding fixed bug in STORMfinderBeta.m For Hao for updates: Fork fast-forward https://github.com/blog/266-fast-forward-your-fork Data Analysis copying files to ProRAID while running splitQVdax … Continue reading →

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Wednesday 09/04/13

Posted on September 4, 2013 by admin

8:15a – 4:50p, 9:30p – 12:00a Discussions discuss project 2 troubleshooting with Hao and Jeff discuss with Jeff filtering STORM data on molecule properties (such as width photon number correlations or average intensity per flash as a function of space). … Continue reading →

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Monday 09/02/13

Posted on September 2, 2013 by admin

8:30a – 6:00p, 8:00p – 11:10p T7 plate reaction planning (?) NEB Quick High Yield T7 template = 10 uL of T7 template (~30-120 ng/uL from spot check) 10 uL NTP buffer mix + 1uL (1/2x) T7 polII mix + … Continue reading →

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