Tag Archives: probe making

Tuesday 05/21/13

Posted on May 21, 2013 by admin

8:30 A – 5:00 P, 7:30 P – 11:45 P Probe making ethanol precipitations Ethanol precipitate B/Y/K/G probes from 2-5 mL scale synthesis (which had poor nicking and extra-large bands). Ethanol precipitate new 4 mL scale B/Y/K/U PCRs with primer-fixed … Continue reading →

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Monday 05/20/13

Posted on May 20, 2013 by admin

9:20 A – 7:00P, 9:30P — 12:20 A Lab meeting visiting post-doc candidate: notes Probe making figure out increased library concentrations for large scale PCR for B/Y/K/G libraries set up 5 mL scale PCR for B/Y/K/G using nick-containing primers Setup … Continue reading →

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Tuesday 05/4/13

Posted on May 14, 2013 by admin

10:00 A – 5:00 P, 9:00 P — 11:45 P Analysis of signal and background in different hybe conditions Discussion with Brian on temperature results Additional notes not yet sent: Hybe conditions that reduce binding to reduce background seem to … Continue reading →

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Protected: more probe making approaches

Posted on May 14, 2013 by admin

There is no excerpt because this is a protected post.

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Saturday 05/11/13

Posted on May 11, 2013 by admin

3:00 P – 8:00 P, 9:30 P – 11:15 P Kinetic modeling Discussion with Jeff sent text books (see email) Lent library book (Feller) to Jeff should write text together? OligoPaint Probe making spin down ethanol precipitating samples of probe … Continue reading →

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Wednesday 05/08/13

Posted on May 8, 2013 by admin

9:30 A — Probe making pool digests aliquot out test DNA: 1 uL digest DNA, 3 uL TBE, 1 uL 6x loading dye + 12x gel green, 5 uL formamide with 5% 5M EDTA Pour new 16% urea PAGE gels … Continue reading →

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Tuesday 05/07/13

Posted on May 7, 2013 by admin

9:25 A -5:50 P Ph FLAG Processing confocal images of Ph data updated ImageShop / ExtractLSM to fix bug in multi-position loader. Ph FLAG discussion / comments: post Literature Pirrotta Insulators target active genes to transcription factories and polycomb-repressed genes … Continue reading →

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Monday 06/05/13

Posted on May 6, 2013 by admin

9:30 A – 7:45 P, 8:45P – 11:55 P Oligopaints Running out gradient gels (one at a time) hotter is better with mis-priming. using 20 bp PPT primer and library specific NP primer: with adapters: Check Ph stains booked confocal … Continue reading →

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Monday 04/15/13

Posted on April 15, 2013 by admin

9:30 A – 8:00 P, 9:00 P – 11:55 P Meetings group meeting (notes) journal club (notes) discussion with Xiaowei (notes) Gels At least 3 ways to screw up gels APS really needs to fresh, (try 140 uL instead of … Continue reading →

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Thursday 04/11/13

Posted on April 11, 2013 by admin

Oligo Paint Probe synthesis Test PCRs at 1 mL scale: Assymetric y-PCR, Y-adapter, no Y-L primer + 1uL of EtOH extracted Y PCR product at 85 ng/uL Assymetric b-PCR, B-adapter + 1 uL of EtOH extracted B PCR product at … Continue reading →

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