Author Archives: admin

Saturday 10/05/13

Posted on October 5, 2013 by admin

(WFA, 8:30a-6:30p) STORM Finish O/N STORM of G05 Set up new STORM of E06-E(Pc) backgroundy? faint spots per cell, too many? Maybe E2 also not working? Some cells have clearer dots? Lets wait to see the topology and do a … Continue reading →

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Friday 10/04/13

Posted on October 4, 2013 by admin

9:30a-4:30p, 7:00p-12:00a Goals Figures for Ajaz! Work on Banff report Start presentation for Stowers order Illumina MiSeq components Order NEB primers PCR Overnight power failures? Tuck auto-restarted in night. Overnight analysis aborted/lost Monet auto-restarted in night. Overnight analysis aborted/lost IR … Continue reading →

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Thursday 10/03/13

Posted on October 3, 2013 by admin

10:00a – 7:00p, 10:00p – 11:50p Goals today prep slides for meeting with XZ 10 cycle PCR with 2 uL of product to add on NEB adapters STORM of more black regions reply to Nicole and Ajaz about Ph Literature … Continue reading →

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Wednesday 10/02/13

Posted on October 2, 2013 by admin

10:00a – 11:30p Goals today repeat plate PCR, work on technique, set up for sequencing STORM pipeline, G10 425kb black. start new small black. work on Banff Report – NO PROGRESS work on slides for XZ meeting – [Full time … Continue reading →

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Tuesday 10/01/13

Posted on October 1, 2013 by admin

9:45a-4:15p, 7:45p – 11:40p Goals Today order new cy3 and 405 primers with new secondary binding sites order new cy5 and cy7 (amine?) secondary oligos for multi-color experiments Work on Banff report sign up for qPCR training at the Bauer … Continue reading →

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Monday 09/30/13

Posted on September 30, 2013 by admin

10:00a – 11:00a, 6:20p – 9:50p Oligo cleanup and concentration measurements for short-RNAs (pure and raw) (1) E02 13 kb green (between R/Y) (2) E06 Epc (12 kb YELLOW) (3) E07 Tou (15 kb YELLOW) (4) E09 RED 8 kb … Continue reading →

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Protected: Coordinating chromatin STORM research goals

Posted on September 27, 2013 by admin

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Friday 09/27/13

Posted on September 27, 2013 by admin

9:00a – 4:30p Multiday Goals Write letter to Ting and company Design D mel. gene library complete ANT-C stains (G1-G5) (need G1 probe to complete). Probe Making PCR cleanups: Large Regions D11 100 kb percicentric Green D12 385 kb Yellow … Continue reading →

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Thursday 9/26/13

Posted on September 26, 2013 by admin

10:00a – 10:40p Goals Laundry email Ting & Co. draft letter on progress on goals on chromatin Analyze existing data for new spots: density, puncta etc. scale factors? (density vs. length or something?) Computer splitdax aborted in night when ProRAID … Continue reading →

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Wednesday 09/25/13

Posted on September 25, 2013 by admin

9:30a – 11:15p STORM stop O/N STORM start copying data to Monet from O/N start spot-finding analysis on yesterday’s O/N splitdax F6 data. G2 data also not analyzed: set up DaoSTORM analysis on Cajal G2 bead data looks sparse — … Continue reading →

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