Monthly Archives: April 2011

fly work

Posted on April 7, 2011 by admin

Flip bcd-3UTR flies into bottle. Flip collection plates, 10:50A, (cact/HbZ and MP09 Hz lines). Sort previous embryo collections, 3x snail in vnd[D38], E(spl) x vnd[D38], E(spl); some already stained see in situs 01/07/10 Flip plates again 1:50, Fix 3:00 P. … Continue reading →

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Wednesday 04/06/11

Posted on April 6, 2011 by admin

9:50 A – 4:00P, 8:50 P – 10:15P Finish O/N confocal run.  Drifted again! despite 1.25 hr warmup.  very upsetting.  (9 embryos, 50 stacks, 330 nm spacing =16.17 um) O/N data analysis still runs out of memory (now finishes 8 … Continue reading →

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Abstract draft for Q-bio

Posted on April 6, 2011 by admin

Quantitative measurements of transcription in single cells have demonstrated a high degree of stochastic variation among clonal cells in copy number of mRNA and protein, resulting from the bursts of molecule synthesis.  These experiments have raised the question of how … Continue reading →

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Tuesday 04/05/11

Posted on April 5, 2011 by admin

8:40 A – 9:15 A, 9:50 A – 7:10 P, 8:30 P — 12:45 A Finish O/N confocal Memory errors still with O/N dot counting (50 is too many in stack?) very few non-sna[D] (not Osp or CyO) mutants in … Continue reading →

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Monday 04/04/11

Posted on April 4, 2011 by admin

10:00A – 7:15 P, 7:45 P – 11:00 P send .ai and .pdf figures to PLoS CB. Clean up fly meeting notes in notebook. Lab server (clusterdraw) down again.  It’s Naaman’s internet or the server, so Naaman has the access … Continue reading →

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Plenary sessions, 04/03/11

Posted on April 3, 2011 by admin

Plenary Session II. Kami Ahmad (Harvard Med School) Nucleosomes. Histone replacement (H3.3). Targeted to active regions. Xnp part of replacement complex. H3 strongly expressed in S phase, H3.3 is constituitive. Constitutive H3 rescues all H3.3 phenotypes (sterility, lethality, etc) Process … Continue reading →

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workshops, 04/02/11

Posted on April 2, 2011 by admin

Pare mRNA counting in Drosophila (McGinnis lab) Recommend acetone permialization. No prot K, need longer hybe. Direct label not as sensitive, much more expensive. More As = better probe (but repeats give background)! Arney et al nut acids 2010. Mounting … Continue reading →

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Techniques Platform, 04/02/11

Posted on April 2, 2011 by admin

Techniques Platform. Perrimon group/ RNAi centers Cell based RNAi screening. Increase in imaging screens (from plate-reader screens). 1-3 million images per std experiment, taken an automatically analyzed. 2-10Tb per screen, currently 35Tb of data. Trying to move to in vivo … Continue reading →

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Platforms 04/02/11

Posted on April 2, 2011 by admin

Regulation of Gene Expression Part I Lehmann lab FACs and RNA-seq to study germline transcription. 7K out 13.8K genes transcribed in germline. Maternal to zygotic transition at stage 8-9 in germ cells. Ovo motif C-GTTA Zeba (dePace Lab) quantitative input … Continue reading →

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platform afternoon 4/1/11

Posted on April 1, 2011 by admin

Maggert lab, (Texas A&M) Ctcf regulates Erna synthesis in nucleuolos spatial and temporal dynamics of homologous recomb reapir.  (Karpen lab) double strand breaks in heterochromatin/ rpt dna. Double strand breaks peak earlier in heterochromatin. after irradiation expansion of hp1a Margarete … Continue reading →

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