Monthly Archives: June 2012

Tuesday 06/19/12

Posted on June 20, 2012 by admin

10:10 A – 6:30P, 8:30P-10:20P Clustering and methods discussion with John John can help with synthesis of BAC probes share matlab scripts for clustering dots and converting to images. (need to iron out bugs and improve annotation). Comments on STORM … Continue reading →

Posted in Summaries | Tagged | Comments Off on Tuesday 06/19/12

Friday 06/15/12

Posted on June 19, 2012 by admin

10:50 A – 7:2oP Collection cages flipped, 10:30A. Troubleshooting but in Dave-program with Hazen on STORM2.  frames randomly dropped from end of movie.  some movies randomly dropped. System is not repeating the movie drop.  Does repeatedly drop the last frame. … Continue reading →

Posted in Summaries | Tagged | Comments Off on Friday 06/15/12

Monday 06/18/12

Posted on June 18, 2012 by admin

working on snail bistability manuscript Comparison of snail patterning to optimal decision making theory for two alternative forced choice (see Holmes et al), see post.  

Posted in Summaries | Tagged , | Comments Off on Monday 06/18/12

Thursday 06/14/12

Posted on June 14, 2012 by admin

10:00A – 7:20P, 8:40 P – 11:30P Start running analysis on MT data from yesterday Flip fly collection cages, 10:10 A 4 new bottles of Pc-GFP and Yw each.  5/28 Pc-GFP bottles ready to transfer to collection cage, should give … Continue reading →

Posted in Fly Work, Summaries | Tagged , | Comments Off on Thursday 06/14/12

Wednesday 06/13/12

Posted on June 14, 2012 by admin

10:00 A-7:00P, 8:00P -11:00P STORM4 training with Yari STORM4 imaging microtubules and 3D beads.  Stage drifts rapidly (including between switching runs) unless joystick is set just right (slight tilt to left). Day 3 labeling embryos.  Joint directly labeled primaries K27me3, … Continue reading →

Posted in Summaries | Tagged | Comments Off on Wednesday 06/13/12

Tuesday 06/12/12

Posted on June 13, 2012 by admin

10:00 A-5:00P, 9:30P – 12:00A Flip collection cages, 10:30A still working on z-calibration bug.  updated write_minimal_dax_ini file seems to be the only script that won’t run correctly.  Putting max_displacement = 1 causes errors in otherwise functional script.  May need to … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Tuesday 06/12/12

Monday 06/11/12

Posted on June 11, 2012 by admin

10:00 A – 7:00P, 8:30P-11:30P Hao lab meeting in situs day 1 (by KE): protein structure between hox targets Fixing AOTF problem with STORM2.  Between beam shape and AOTF, no usable light is passed. AOTF removed from table. analyze probe … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Monday 06/11/12

Sunday 06/10/12

Posted on June 10, 2012 by admin

4:00P – 7:30 P old-version of auto z-cal converges effectively on old and new data. Start making slides for meeting with Xiaowei next week. New probes to make: lab, scr, bxd, mir-10, Ama, CG5220 + CG3995, CG10324 + CG 10326, … Continue reading →

Posted in Summaries | Tagged | Comments Off on Sunday 06/10/12

Saturday 09/06/12

Posted on June 9, 2012 by admin

11:45 A – 7:15P, 8:30P – 10:30P Staining microtubules in 4 colors for imaging control (with help form Graham) Finish O/N STORM run of Pc-GFP with before and after 3D beads and z-calibration movies Finish staining no primary controls of … Continue reading →

Posted in Summaries | Tagged | Comments Off on Saturday 09/06/12

Friday 06/08/12

Posted on June 8, 2012 by admin

10:00 -8:00P, 9:00P -11:20P Flip fly cages Finish clean up O/N STORM run.  Coopying files from Alistair5 to Alistair4. move in situ probe-test slides into boxes.  (Move archive boxes into 4C bin to make more space in 4C drawer?) 11:00 … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Friday 06/08/12