Monthly Archives: November 2012

Thursday 11/07/12

Posted on November 8, 2012 by admin

9:30 A -8:00P, 9:30-11:00P check STORM.  — run finished without losing focus. Summary of inv run:  Unfortunately 514 doesn’t work either as a double label. inv-Epc male a lot of promising double spots, but it’s a litter harder to sort … Continue reading →

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Wednesday 11/06/12

Posted on November 7, 2012 by admin

9:45 A – 8:30 P write to ML about bistability write to Bender about Hox model.  — apparently email contact is not recommended.  Try phone tomorrow. write summary of Francis suggestions remove medium from S2 cells, add fresh medium.  Reduce … Continue reading →

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Protected: Francis Lab Meeting feedback

Posted on November 7, 2012 by admin

There is no excerpt because this is a protected post.

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Protected: snail project further experiments

Posted on November 7, 2012 by admin

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Tuesday 11/06/12

Posted on November 6, 2012 by admin

9:15 A – 9:00P, 10:ooP-11:00P (vote, 8:45A) Washing out probe from on slide DFISH.  hot wash at 60C -forgot for 2hrs (oops!) stain with Hoechst and WGA-488 Working on presentation for Francis group meeting STORM characterizing embryo background (see images) … Continue reading →

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Monday 11/05/12

Posted on November 5, 2012 by admin

9:30A -10:00P Overview of today Flip sim[D] plate (9:30A) Colenso Lab meeting Age sim[D] plate and 3 MTD x SCM plates Fix sim[D] and SCM embryos.  Should be ready for in situs Working on Francis lab group meeting presentation / … Continue reading →

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Sunday 11/04/12

Posted on November 4, 2012 by admin

11:00 A – 8:00P Cell culture and cell labeling Start washing out direct-labeled primaries from cell-culture plates. Split cells, toss old plates. plate cells on round cover glass in 12 well pates.  See if these adhere any better for FISH-on … Continue reading →

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Saturday 11/03/12

Posted on November 3, 2012 by admin

10:45 A – Writing Write BJ review Drosophila PWMs TF binding motifs FlyFactor Ecoli TF binding + PWMs here (might be something better) Finish first draft through of enhancer integration section.  On to promoters next. DFISH on cell culture remove … Continue reading →

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Friday 11/02/12

Posted on November 2, 2012 by admin

9:30 A – 7:48 PM, 10:50 – 11:45PM Fly work Flip collection plates on MTD x SCM, MTD, and sim[D], 9:30A Flip collection plates, sim[D] 1:15  P Collect virgin Act5-Gal4, MTDs, and sim/Espl.  Sort sim/Espl against humoral bristles. New crosses … Continue reading →

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Thursday 11/01/12

Posted on November 1, 2012 by admin

9:30 A – 7:30P, 8:30P- 12:00A Software install on STORM2.  Hal can’t detect drivers for national instruments panel. Overview of software install: see post Fly work Collect virgins Sort and cross Espl/sim flies Clear MTDs into 2 new bottles, move … Continue reading →

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