Monthly Archives: December 2012

Thursday 12/06/12

Posted on December 6, 2012 by admin

9:45 A – 7:30 – 11:55P Troubleshooting antibodies with polytene H3K9me2 failed completely on polytene cells.  Dm01 control labeled in parallel looks bright and clean (better than direct labeled Dm01). Polytene labeling today: testing H3K27me3 (see if polytene chromosomes label … Continue reading →

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Wednesday 12/05/12

Posted on December 5, 2012 by admin

9:30 A  – 7:40P, 9:00P – 12:00A Confocal dot distances Finish O/N confocal run of Ubx-AbdA data Probe making sent order to LC Sciences for chromatin types On why previous probe might have failed:  over drying? — DNA and RNA … Continue reading →

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Tuesday 12/04/12

Posted on December 4, 2012 by admin

9:40 A – 7:45P, 9:00P – 1:00A Dfd/tub analysis Break down O/N confocal run 3 channels, speed 6, 22 positions, 17.5 finished in 11 hour run.  contact zeiss rep Casey about changing channels between z-stacks rather than every section, should … Continue reading →

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Code updates

Posted on December 4, 2012 by admin

minor tweak to TiffViewer m MatlabPhotoshop/TiffViewer.m minor tweak to TiffViewer split out assign dots to nuclei code from unsupervised dot finding script, so it can be called by other scripts. + AssignDots.m split out assign dots to nuclei code from … Continue reading →

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Monday 12/03/12

Posted on December 3, 2012 by admin

8:45 A — 7:30 P, 8:45P – 12:40 A Confocal data Finish O/N confocal run transfer files modify imviewer LSM to automatically detect all .lsm files in a folder and convert them all to TIF / labeled stacks of TIFs … Continue reading →

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Sunday 12/02/12

Posted on December 2, 2012 by admin

10:30 A – 7:30P, 8:30P – 11:45P Lab meeting prep collecting dual labeled images to show attempts with flanking probes. PCR Test run gels from Espl screening.  Gel 1 from BioXAct (3A and 4A, see legend in yesterday’s post), numerous … Continue reading →

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Saturday 12/01/12

Posted on December 1, 2012 by admin

cell staining control worked, 50kbp probe did not. However, 50kbp probe does show weak global nuclear staining immediately after removing probe, which is not visible in control channels. hot washes started 60C SSCT hot washes dramatically reduce signal from control … Continue reading →

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