Tag Archives: STORM

Tuesday 07/31/12

Posted on July 31, 2012 by admin

11:00 A – 7:40P, 8:20P-11:00P (volleyball practice 9A-11A) Flip fly cages KE fix embryos 2-6 hrs old (Pc-GFP and YW) Reorganizing text for kinetics paper: homogeneity, dynamics and kinetics, dosage compensation. Adapt figure_sna_dynamics to show spatial profiles over time. STORM centromere … Continue reading →

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Friday 07/27/12

Posted on July 27, 2012 by admin

9:40 A – 7:40, 9:00P – 12:30A Finished confocal run, 8.5 embryos finished at speed 5 by 10A (run started at 10P). Previous user canceled 710 time (again).  Need to check before starting or stopping a run. Over-etched first K27 … Continue reading →

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Monday 07/16/12

Posted on July 16, 2012 by admin

9:40A – 5:00P Etching s6: 5E-dig, AbdB-bio.  K27-647 looks good on Turnkey.  Etched 22 min in second use of sodium ethoxide (at 22C for 2 weeks, now dark brown). Review knife care with KE.  (Trimming knife was badly chipped during … Continue reading →

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Tuesday 07/10/12

Posted on July 10, 2012 by admin

10:10 A – 5:15 P, 10:30P – 12:45A etching H3K4 4-color coverslip 3 in new sodium ethoxide (pale yellow color) for 13 min = mostly etched, resin definetely in the doubled over sections, some patches on single embryos.  clear bur … Continue reading →

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Thursday 07/5/12

Posted on July 5, 2012 by admin

9:50A – 7:15P, 9:00P – 10:45P check nascent transcripts of AbdA (red) and Antp (green) on yw and Pc O/N short-fix embryos.  No obvious staining on turnkey. Should check 6/11 embryos as positive controls and 6/25 embryos to see effect … Continue reading →

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Tuesday 07/03/12

Posted on July 3, 2012 by admin

10:00 A – 7:10P, 8:45P-11:00P Sent version 4 of snail dynamics manuscript to ML Flip collection cages. 12:30 PM fix Pc-GFP and yw embryos.  15 min fix.  Rehydrating embryos to test protein stains. Image sections on Turnkey.  sections substantially non-flat. … Continue reading →

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Monday 06/25/12

Posted on June 25, 2012 by admin

9:45 A – 7:00P, 9:30P – 11:45P Need new collection cages FISH + K27 on O/N embryos day 1 Flipped 3x Mat Gal4 cages. Running Z-calibration analysis on Suz647 data (with Quadview) Collecting new z-cal bead data in 512 and … Continue reading →

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Friday 06/22/12

Posted on June 23, 2012 by admin

10:30 A – 5:30, 8:30-11:30P Make new bead slide with 40 nm 540 beads and 100 nm IR beads. Finish O/N STORM run and shoot beads. Fixed issue with 3D bead mapping not mapping IR and Vis 647 channels together. … Continue reading →

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Thursday 06/21/12

Posted on June 21, 2012 by admin

2:00P — 8:30P Calibrating new bead imaging: 1:20 then 1:500 540 beads + 1:500 Q-dots-705.  Too dense. Dilute a further 1:500.  Q dots good density.  Blinky, excited best by 405 or 488.  Look good in 647, not really visible in 750 … Continue reading →

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Tuesday 06/19/12

Posted on June 20, 2012 by admin

10:10 A – 6:30P, 8:30P-10:20P Clustering and methods discussion with John John can help with synthesis of BAC probes share matlab scripts for clustering dots and converting to images. (need to iron out bugs and improve annotation). Comments on STORM … Continue reading →

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