Tag Archives: coding

Monday 03/19/12

Posted on March 19, 2012 by admin

9:40A – 11:55 P Lab meeting presentation: Jeff Moffit 1 hr RT O/N primaries washing out O/N primary antibody labels ACS draft app to Matt Fly meeting receipts organized, sent to Matt Promoter synchrony paper feedback for Mounia and Jacques … Continue reading →

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Friday 03/16/12

Posted on March 16, 2012 by admin

9:00 A — 9:30P Finish O/N confocal run, save data Projecting data in Matlab Make new and autoclave: 2x YT aliquot IPTG and X-Gal Transform and plate competent cells, 5:30 plates at 37C O/N.  300 uL per plate PCR with … Continue reading →

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Saturday 03/03/12

Posted on March 3, 2012 by admin

11:00A – 7:00 P Overnight run of Psc H3K27 aborted after ~23 sections (235 Gb) . Moving files from Alistair2 (G:\) to Alistair3 (K:\) to mobilize Alistair2 for data collection tonight. Can copy files over local intranet quickly from G … Continue reading →

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Friday 03/02/12

Posted on March 2, 2012 by admin

10:05 A -1:00 A Flip new PcG shRNA lines. Enter into stocks. Screen MP12 rescue crosses.  set up 6 new crosses, 3 with bright eyes might be homozygous MP12. 1 vial with straight wings tossed and 1 vial with Sp … Continue reading →

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Tuesday 02/28/12

Posted on February 28, 2012 by admin

9:45 A – 12:45 A Evaluating 3D fitting: section joins do not look smooth Wrote new script (list2tif3d_weq.m) which distributes samples uniformly in z.  Samples near the focal plane are best resolved and can have higher z-resolution. Current approach still … Continue reading →

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Monday 02/27/12

Posted on February 27, 2012 by admin

9:40 A – 7:40P, 8:30P – 12:15P Setup sample S2 33o nm (4 color) and Psc-hox 330 nm (2 color) to run through Insight. Sectioning: 2 slides of 2H and 2 slides of rad21 cut.  slides 2H react strongly to … Continue reading →

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Wednesday 02/10/12

Posted on February 1, 2012 by admin

11:00A – 7:30P, 8:30P-11:30P Flip collection cages 11:20A Order Primers for Taf1, aTub84B, m13F/R Confocal: STORM prepped slides.  Both 1hr and 35 min samples are substantially over etched, most of sample is gone.  Try 20 min next (?). Confocal: MP08 … Continue reading →

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Zhuang Lab VPN from linux

Posted on January 30, 2012 by admin

Install Remmina Remote Desktop Client (also available through Ubuntu Software Central). Download Harvard’s Cisco Client (.sh file) from: https://core-vpn-1-gw.fas.harvard.edu/CACHE/stc/4/binaries/vpnsetup.sh Alternatively follow the connect to vpn link from the Harvard vpn site.  This tries to launch a web/java version of Cisco.  If that fails to … Continue reading →

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Sunday 01/29/12

Posted on January 29, 2012 by admin

10:30 A – 2P, 4P – 12:30A working on snail simulations finally found bug in simulation: conn_map missused – entries are width of connections, not indices of cells. configured remote connection on Linux laptop to vpn onto Monet (see post … Continue reading →

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Saturday 01/28/12

Posted on January 28, 2012 by admin

11:00 A confocal O/N scans still running (22/26 embryos, started at 10 P, 35 sections, speed 5).  Extended run time until 3P. Literature search for good Drosophila Cohesin antibodies wrote to Dale Dorsett, might have Rad21 antibody to send, recontact … Continue reading →

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