Tag Archives: writing

Friday 08/24/12

Posted on August 24, 2012 by admin

10:15 A – 9:30P running analysis on images sent by Mounia.   see post4160. Write up snail analysis updates (see post). Trying to fix lsm format error on image 13 and later of m106a.  Unable to ID parse error. Write … Continue reading →

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Tuesday 07/31/12

Posted on July 31, 2012 by admin

11:00 A – 7:40P, 8:20P-11:00P (volleyball practice 9A-11A) Flip fly cages KE fix embryos 2-6 hrs old (Pc-GFP and YW) Reorganizing text for kinetics paper: homogeneity, dynamics and kinetics, dosage compensation. Adapt figure_sna_dynamics to show spatial profiles over time. STORM centromere … Continue reading →

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Monday 07/30/12

Posted on July 30, 2012 by admin

9:50 A – 5:00 P, 9:30P – 10:45p Jiang lab meeting difficulty connecting to notebook 710 confocal all sessions canceled, new 780 being installed this week.  Requested training on ELYRA which also has a 710 (and maybe a programable stage?) … Continue reading →

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Friday 07/13/12

Posted on July 13, 2012 by admin

9:50 A – 7:00P Submit kinetics paper John recommends 3 into 20 dilution of BAC probe, stain on slide in a sandwich between coverglass and a slide.  Use heat block with insert upside down for hybrdization clean up Snail Doc … Continue reading →

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Monday 07/09/12

Posted on July 9, 2012 by admin

9:50 A – 6:50 P remove slide 3 of H3K4-section from 60C heat block, add to slides. Hazen Lab meeting 3D viewing in Insight:  ctrl+shift+x, select ‘no’, ctrl+x gives 3D viewer.  Need to have color shading as percentage toggled on … Continue reading →

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Friday 07/06/12

Posted on July 6, 2012 by admin

10:10 A – 7:30P, 9:00P -11:15P Flip fly cages, 10:10A Revisions of dynamics paper, revised, updated to v7. compare to NM requirements.  Version and comparison sent back to ML.  May need to combine figures and prune text. Take embryos out … Continue reading →

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Thursday 07/5/12

Posted on July 5, 2012 by admin

9:50A – 7:15P, 9:00P – 10:45P check nascent transcripts of AbdA (red) and Antp (green) on yw and Pc O/N short-fix embryos.  No obvious staining on turnkey. Should check 6/11 embryos as positive controls and 6/25 embryos to see effect … Continue reading →

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Tuesday 07/03/12

Posted on July 3, 2012 by admin

10:00 A – 7:10P, 8:45P-11:00P Sent version 4 of snail dynamics manuscript to ML Flip collection cages. 12:30 PM fix Pc-GFP and yw embryos.  15 min fix.  Rehydrating embryos to test protein stains. Image sections on Turnkey.  sections substantially non-flat. … Continue reading →

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Monday 07/02/12

Posted on July 2, 2012 by admin

10:15 A – 6:30P, 8:30-9:30P Flip collection cages, 10:15 A Finishing embedding of SuZ labeled embryos HCBI access expired. Need to fill out paperwork again to restore access. Analyzing previous Pc-GFP images.  z-calibration not as good as recent attempts with … Continue reading →

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Wednesday 06/27/12

Posted on June 27, 2012 by admin

10:10 A – 11:00P in situs day 3 washing out and post-fixing Pc labeled primaries write to Nicole Follmer about antibodies lit review: piRNA in C elegans multigenerational epigenetic memory.  LacR facilitated diffusion.  DNA sequence dependent compartmentalization and silencing an … Continue reading →

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