Tag Archives: confocal

Wednesday 01/25/12

Posted on January 25, 2012 by admin

10:20 A Analyzing confocal images from testing antibodies WGA-488, H3K27me3-Cy3B (red), H3K4me3-Cy5 (blue) running LSM files through imviewer install TeXMaker on desktop working on fellowship applications BIRS proposal for 2013 5 day workshop rejected.  Contacted co-organizers to re-stratagize. Writing imviewer … Continue reading →

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Tuesday 01/24/12

Posted on January 24, 2012 by admin

10:00A – 1:00 A Finish v5 of JCC draft, sent to CB and DB. New version of figure for JCC app. 2 new sna[D] bottles for virgin collection to remake MP12 rescue line. DB MP12 flies flipped back to vial … Continue reading →

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Saturday 01/21/12

Posted on January 21, 2012 by admin

8:45 A — 2:00 P in situs moved through resin, dense collection of embryos 10 embedding capsules of embryos made for each of the 4 in situ tubes (+4 of tube 1 by KE for practice). in situ embryos baking … Continue reading →

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Friday 01/20/12

Posted on January 20, 2012 by admin

10:10 A -11:00 P labeling primary anti-histone antibodies (see Alistair_STORM_dyes log): pan-H3, H3K4me3, H3K27me3, and H3K27ac.  Need to filter Ac and K27 through column before binding.  Also filtered H3 and K4 — dilutes sample sufficiently to allow for multiple subsequent … Continue reading →

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Monday 01-16-12

Posted on January 16, 2012 by admin

6:30P – 11:00P working on figures for fellowship apps images of open and closed engrailed loci confocal, engrailed slides 6-20-11.  imaging slide 4 ON. comparing probe labeling efficiencies:  tou-dnp does not work, tou-flu weak and backgroundy.  E(Pc)-dig is strong, en-bio and … Continue reading →

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STORM imaging progress update

Posted on January 14, 2012 by admin

Review of recent progress on multicolor STORM imaging of gene regulation. WGA labeling   membrane in 100 nm section.  little Hb detected (red stain mostly background). en-dig stains well with H3K27.  just need to cut it better. AbdA (red) and Ubx (green) … Continue reading →

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Saturday 01-14-12

Posted on January 14, 2012 by admin

11:00A – 7:50 P No engrailed stain No evidence that short fix, antibody label, then in situ, actually labels mRNA efficiently. No hb-mRNA detected. attempted imaging of en-dig (cut yesterday) 50 min etching removed all cells, only bits of yolk … Continue reading →

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Friday 01/13/12

Posted on January 13, 2012 by admin

10:10 A -8:00P Start etching K4 Flip MP08 cage (10:40A) New MP08 cage cut eng-dig sections 3 – 4:30 cut new Ftz, eng labeled embryos 4:30 -6p, section’s of previously trimmed block do not hold together well. cut selection of … Continue reading →

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hunchback and bcd antibody testing

Posted on January 12, 2012 by admin

some quick checks looking at dynamically refinement of hb gradient. Hb (green) and Bcd (red) double stained (early cc13?)         zoom , showing subnuclear localization patterns.           showing very gradual hb transition   … Continue reading →

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Saturday 12-17-11

Posted on December 17, 2011 by admin

12:00P – 1:00 P, 8:45P – 10:20 P O/N confocal only finished 12 embryos between 8p and noon, extended run to finish remaining 4 selected on slide. mount all MP08.  mount subsets of embryos: eng-dig, eng-fl, antp+ubx, AbdA+ubx. remaining embryos … Continue reading →

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