Monthly Archives: May 2011

probe making: chromatin condensation

Posted on May 25, 2011 by admin

Linearized templates for: inv A, E(Pc) A, tou A, en A (in order). Gel extracted and purified. All concentrations ~20 ng/uL.             Synthesis setup: [everything with T7 :), all templates same conc. ]. 20x master: 100 … Continue reading →

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Tuesday 05/24/11

Posted on May 24, 2011 by admin

Interview at Janelia 8:30 – 9:30 am Breakfast with Kevin Moses – Bob’s Pub 9:30 am Talk setup in Auditorium 10:00 am Talk in Auditorium “Molecular Mechanisms of Precise Gene Regulation in Drosophila” 10:30 am Break 10:45 am Panel Session … Continue reading →

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Monday 05/23/11

Posted on May 23, 2011 by admin

Fly to Janelia Meeting with Sean Eddy Final prep of seminar talk

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Sunday 05/22/11

Posted on May 22, 2011 by admin

1:30P-7:10P, 8:20P-10:30P Collect virgin Gal4-bcd-3utr mount slides from in situs Remove Tuxera.  Install G-RAID and convert to NTFS file format. Sequences (with m13R): inv A: +/+/gene is +, T7.   inv B +/-/+, SP6. E(Pc) A +/+/+ T7.  E(Pc) B … Continue reading →

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Thursday 05/19/11

Posted on May 19, 2011 by admin

10:30 A — 1:00P, 4:30-5:00P, 11:00P-12:30A in situs day 3 collect virgin bcd-gal4 (AM PM) embryos at 4C ON

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Wednesday 05/18/11

Posted on May 18, 2011 by admin

10:45 A — 6:00P, 9:30P – 11:55P Flip collection cages, 11:00A, Flip cages, 3:00P.  fix embryos 3:30P warming hybe solution, in situs day 2. Collect virgins (bcd-Gal4) Flip cages 6:00P Fix embryos 10:00P (MP05, MP06, MP09, MP10, MP02, bcd 1x, … Continue reading →

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Tuesday 05/17/11

Posted on May 17, 2011 by admin

11:00 A – 2:00P, 10:00P – 12:00A collect virgins G4B in situs day 1B: add probes minipreps (inv en E(Pc) tou) collect virgin G4B send samples for sequencing

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Monday 05/16/11

Posted on May 16, 2011 by admin

10:30 A – 7:00P, 8:00P – 12:00A Flip collection cages 10:40A Collect virgin Gal4-bcd-3UTR and BAC4 early and late rescues. submit progress report to ML set up new cages: MP05 Hz, MP09 Hz. synthesize DNP probes and fluorescene probes y1, … Continue reading →

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progress report

Posted on May 16, 2011 by admin

Alistair- One month ago, April 13, you indicated the following key experiments: -quantify sog lacZ mRNA levels with one vs. two enhancers -sna BAC – shadow enhancer X sna-/sna- genetic complementation* -Hb BAC yellow series.  compare fluctuations in de novo … Continue reading →

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Sunday 05/15/11

Posted on May 15, 2011 by admin

4:30 P, 8:30P – 11:45 P Flip cages 4:30P Fix embryos 9:30P (MP10, MP02, MP06, bcd 1x, bcd 6x) Clear bcd-Gal4 flies for virgin collection.

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