Monthly Archives: February 2012

PcG bodies

Posted on February 24, 2012 by admin

PcG bodies of Pho and Psc.

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STORM 2-24-12

Posted on February 24, 2012 by admin

Laser powers 750: fix to 100 mW 647: 175 mW 561 (at 200 mW activation) fix to 150 mW 488:  200 mW 405: 1.5 mW Imaged S6 500 nm, single section with hox expression dot. Arraying sample of S7, engrailed … Continue reading →

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Thursday 02/23/12

Posted on February 23, 2012 by admin

10:05 A – 12:10A PcG and Bcd staining embryos incubating in primary at RT for final hour. Flip fly plates Discussion with John about projects Need to look up Drosophila repeat sequences for probe testing. in situs day 1 on … Continue reading →

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Wednesday 02/22/12

Posted on February 22, 2012 by admin

9:30 A – 8:50 PM Sectioning: 300 nm. trapazoids work better, long edge at bottom. Cut 3 coverslips of S6.  Two at 330 nm, one at 100 nm. Coverslip 1 at 330 nm is tiled from upper left to lower … Continue reading →

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STORM 02/21/12

Posted on February 22, 2012 by admin

  Laser powers 750: 102 mW 647: 200 mW 561: 150 mW 488: 200 mW 405: 1.5 mW imaging slide S7 750 looks pretty good, Dm01 better in young embryos. Other channels look like resin is still strong in outline. … Continue reading →

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confocal test summary

Posted on February 22, 2012 by admin

confocal of:  

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Tuesday 02/21/2012

Posted on February 21, 2012 by admin

9:40 A – 12:15 A Flip embryo cages, 9:45A Flip collection cages: 3:30P Fix embryos: 4:30 P Confocal images of latest stains: PcGs with histones and en or hox complex:  hot wash was too long (4.5hrs), most in situ signal … Continue reading →

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Monday 02/20/12

Posted on February 20, 2012 by admin

9:45 A — 10:00 P Transfer embryos into 30% resin Group meeting / BPS practice talks Transfer embryos into 50% resin New collection cages: 4x snail, yw, and MP09s. embryos into 70% resin.  3 hrs, then 100% resin. images uploaded … Continue reading →

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Saturday 02/19/2012

Posted on February 20, 2012 by admin

10:00A – 5:00P washing out final antibody from in situs. embryos moved to ethanol WGA staining Hox in situs 1:10 set up to try cutting thicker samples.  Using Yari’s new walled area for microtome. select Images for Cell feature on … Continue reading →

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Friday 02/17/12

Posted on February 19, 2012 by admin

10:10 A — 6P, 8P-12:30P Finish O/N STORM run Analyze STORM data collect virgins working on alignment for STORM data.  Rigid alignment seems better for the moment. staining embryos, in situs day 3. New scheme for PcG and histone doubles: … Continue reading →

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