Monthly Archives: March 2012

Wednesday 03/21/12

Posted on March 21, 2012 by admin

12:00P – 9:30P (9A-12P lab volleyball practice) in situs day 2 protein stains wash out primaries, post-fix, add secondaries. started new page, see record: Kill old bottles of MP09, yw.  Need to set up new bottles STORM imaging of 3Mb … Continue reading →

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Tuesday 03/20/12

Posted on March 20, 2012 by admin

10:00 A – 8:00P, 9:30P-10:30P In situs day 1 Flip fly cages, 10:30A Attempted sodium borohydride treatment of half of samples.  Left sealed on shaker — vials exploded, rocket off of shacker from underneath tin foil cover half way across … Continue reading →

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Monday 03/19/12

Posted on March 19, 2012 by admin

9:40A – 11:55 P Lab meeting presentation: Jeff Moffit 1 hr RT O/N primaries washing out O/N primary antibody labels ACS draft app to Matt Fly meeting receipts organized, sent to Matt Promoter synchrony paper feedback for Mounia and Jacques … Continue reading →

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STORM3

Posted on March 19, 2012 by admin

STORM 3 setup notes 561 line: Com 8, up to 100 mW at detector when running at 300 mW at laser.  Controlled by AOTF 656 red line.  Power switch on tabel, modulated by AOTF.  up 100 mW at detector: mirrors … Continue reading →

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Sunday 03/18/12

Posted on March 18, 2012 by admin

11:00 A – 8:45 P No colonies on plates Finish O/N STORM run.  Run failed at movie 121, unknown error message. attempt bead feducial drift correction: polysterene beads good autofluoresnce in all but 750, but bleach fast. 405 beads not … Continue reading →

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Saturday 03/17/12

Posted on March 17, 2012 by admin

11:00 A – 10:00P Experimenting with heat fixing embryos working on ACS application filter extract new tracer beads.  Initial test suggests still contains raw dyes.  Tried spin. No obvious colonies on plates.  Back at 37C O/N. STORM imaging of Pho … Continue reading →

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Friday 03/16/12

Posted on March 16, 2012 by admin

9:00 A — 9:30P Finish O/N confocal run, save data Projecting data in Matlab Make new and autoclave: 2x YT aliquot IPTG and X-Gal Transform and plate competent cells, 5:30 plates at 37C O/N.  300 uL per plate PCR with … Continue reading →

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Thursday 03/15/12

Posted on March 15, 2012 by admin

9:45 A – 9:30P minor z drift issues still observed in STORM run. Time to take vertical beads for z-fit.  at 1:1000 density is a bit low. try 1:500 using ND 2-3 for z-fit bead images. Might be saturated?  Need … Continue reading →

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STORM run 03-14-12

Posted on March 14, 2012 by admin

sample Psc, K27 40 min etch, 330nm sections Laser Powers: 750=110mW, 647=110mW, 561 = 150mW, 488=150mW Start time = 7:25PM 561 very dense switching with 50% 405 power still at 35,000 frames.  stop it there. Occassional section has focal plane … Continue reading →

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Wednesday 03/14/12

Posted on March 14, 2012 by admin

11:00A – 12:30 A Complete and mail DR app. Cloning! Dilute primers.  PCR amplify: Taf1, aTub84b, Neu3 from MP, en from BAC7 Modencode chromatin tracks failing to load again.  Wrote to modencode help. Working on drift correction.  Wrote drift simulation … Continue reading →

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