Monthly Archives: April 2012

Tuesday 04/10/12

Posted on April 10, 2012 by admin

10:00A -5:00P, 10:00P – 11:00P Take PCRs out of thermocycler Take bead images from STORM run Check plates from transformation.  Pick colonies tonight if good. All plates have lots of colonies, including negative control :(.  Test gel of PCR cloning

Posted in probe and plasmid building, Summaries | Tagged | Comments Off on Tuesday 04/10/12

Protected: PcGs in later mitotic stages

Posted on April 10, 2012 by admin

There is no excerpt because this is a protected post.

Posted in Chromatin | Tagged | Comments Off on Protected: PcGs in later mitotic stages

Monday 04/09/12

Posted on April 9, 2012 by admin

10:00 A – 10:30 P Lab meeting (Doory) Fix alignment of 750 laser with Colenso’s help Freeze Bcd-GFP and eve-GFP bottles. NuGru presentation (Andi) Working on snail manuscript supplemental figure captions Download ModEncode data for H3K4me3 and H3K27ac Etch and … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Monday 04/09/12

Protected: 4x snail data

Posted on April 9, 2012 by admin

There is no excerpt because this is a protected post.

Posted in snail patterning | Tagged , | Comments Off on Protected: 4x snail data

Sunday 04/08/12

Posted on April 8, 2012 by admin

4:30 P – 8:30 P 4x snail data looks excellent.  See post. working on snail manuscript supplement

Posted in Summaries | Tagged | Comments Off on Sunday 04/08/12

Saturday 04/07/12

Posted on April 7, 2012 by admin

10:00 A – 7:15 P Confocal: embedded en-E(Pc)-tou-H3 sample does indeed have good intronic staining.  Not many 330 nm sections happen to contain the locus.  Plenty but still a minority of cells are clearly transcribing E(Pc).    So most sections … Continue reading →

Posted in Summaries | Tagged , | Comments Off on Saturday 04/07/12

Friday 04/06/12

Posted on April 6, 2012 by admin

1:00P – 8:30 P (morning lab volleyball practice) Finish reviewing manuscript, write up review for submission.  Still need final read-over and submit. Revising snail manuscript, working through results section. Gel extracting probe templates with KE. Confocal imaging, test stains.  Pre-wash … Continue reading →

Posted in Summaries | Comments Off on Friday 04/06/12

Thursday 04/05/12

Posted on April 5, 2012 by admin

10:00 A — 8:30 P Take GFP embryos out of 4C, incubating at RT heating up 2x SSCT for hot wash mounted oligo-paint embryos before and after washes (with WGA-488 for post-wash embryos).  Remaining embryos at 4C Cut sections of … Continue reading →

Posted in Summaries | Tagged , , , | Comments Off on Thursday 04/05/12

Wednesday 04/04/12

Posted on April 4, 2012 by admin

9:35 A – 7:30, 9:30 – 10:50 P wash out O/N antibodies Flip fly collection plates 10:10A Request more time to write review Flip expanding Gal4-Bcd3’UTR lines:  growing slowly only 2 bottles worth O/N collection plates of en-GFP, Bcd-GFP, Pc-GFP … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Wednesday 04/04/12

Tuesday 04/03/12

Posted on April 3, 2012 by admin

10:00 A – 11:00P washing out primary antibodies (after 10 hrs at 4C) maybe should do longer, return to 1 hr RT followed by O/N 4C? We’ll see how these ones look. Confocal images of embedded embryos for sectioning today … Continue reading →

Posted in Summaries | Tagged , , | Comments Off on Tuesday 04/03/12